Human RelA (p65) NF-kappa B (NF-kB, Nuclear Factor-kappa B) ELISA Assay Kit

Manufacturer Fivephoton
Type Elisa-Kit
Specific against other
Amount 96T
Item no. NFKB-ELISA(h)
Eclass 6.1 32160605
Eclass 9.0 32160605
Detects total human p65(RELA)
Affinity purified polyclonal capture antibody, with polyclonal HRP-conjugated detection antibody
Standard Peptide Concentration: 2250 pg/ml
Assay Range: 100 - 2000 pg/ml
HRP-Colorimetric detection at 450nM
Strip plate format
Rapid assay: 1 hr total incubation time at 37C (i.e. with plate and HRP-detection Ab).
Nuclear-Cytoplasm Isolation Kit offered as an option to separately quantitate nuclear and cytoplasmic p65 as a method to estimate levels of NF-kappa B activation
Separate p65 ELISA kits are offered for human, mouse and rat
The RelA p65 NF-kB (NF-kappa B, NF-{kappa}B) ELISA kit employs the sandwich ELISA(96T) format to quantitate NF-kB p65 protein levels. The 96-well strip plate is coated with an affinity purified polyclonal capture antibody selective to the RelA (p65) subunit of NF-kB. After addition of sample, incubation and wash, a second polyclonal anti-RelA (p65) HRP-conjugated antibody is added to each well. Color reagents are added to generate a colorimetric reaction, which corresponds to the concentration of RelA (p65) in the sample.

A Nuclear-Cytoplasmic fractionation kit is offered to partition cytoplasmic and nuclear p65. p65(RelA) in the nuclear and cytoplosmic fractions can be directly measured with this kit to provide an estimate of NF-kappa B activation by calculation of the nuclear p65 relative to cytoplasmic p65 concentration ratio.
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Amount: 96T
Available: In stock
Listprice: €817.80
Price: €817.80

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