NF-kappa B, NF-kB, NF-kappaB, NF-{kappa}B, Nuclear Factor-Kappa B, Activation Assay Kit (NFKB-2)

Manufacturer Fivephoton
Category Products / Kits
Type Kit
Specific against other
Amount 1 kit
ArtNr NFKB-2
Eclass 6.1 32161000
Eclass 9.0 32161090
Available
Highlights
Measure NF-kappa B activation in a wide range of mammalian species (human, mouse, rat, hamster, bovine, equine, porcine, canine) using cells or tissues.
Suitable for 50 to 150 NF-kappa B activation assays based on one assay per semi-confluent 10 cm cell culture dish, or a well in a 6-well culture dish, respectively.
Offers a more reliable, selective and specific quantitative analysis compared to ELISA formats.
Independently validated in multiple published investigations.
Background
Following stimuli that elicit the NF-kB pathway, the IkB inhibitory subunit of the NF-kappa B complex is phosphorylated, ubiquitinated and degraded, exposing nuclear targeting signals in the transcription factor subunits of the NF-kappa B complex. The NF-kappa B transcription factor subunits, p65 and p50, with exposed nuclear targeting signals, then translocate into the nucleus. This NF-kappa B assay kit provides an accurate quantitative estimate of NF-kappaB activation by measuring p65 (RelA) protein that has been translocated to the nucleus.
References
Baltimore D. Discovering NF-kappaB. 2009 Cold Spring Harb Perspect Biol. Jul; 1(1): a000026.

Bellarosa D, Binaschi M, Maggi CA, Goso C. 2005. Sabarubicin- (MEN 10755) and paclitaxel show different kinetics in nuclear factor-kappaB (NF-kB) activation: effect of parthenolide on their cytotoxicity. Anticancer Res 25: 2119-28.

Campo GM, Avenoso A, Campo S, D'Ascola A, Traina P, Calatroni A. 2008. Chondroitin-4-sulphate inhibits NF-kB translocation and caspase activation in collagen-induced arthritis in mice. Osteoarthritis Cartilage 16: 1474-83.

Cascinu S, Berardi R, Salvagni S, Beretta GD, Catalano V, et al. 2008. A combination of gefitinib and FOLFOX-4 as first-line treatment in advanced colorectal cancer patients. A GISCAD multicentre phase II study including a biological analysis of EGFR overexpression, amplification and NF-kB activation. Br J Cancer 98: 71-6.

Chen HH, Chen TW, Lin H. 2009. Prostacyclin-induced peroxisome proliferator-activated receptor-alpha translocation attenuates NF-kappaB and TNFalpha activation after renal ischemia-reperfusion injury. Am J Physiol Renal Physiol 297: F1109-18.

Ea CK, Baltimore D. Regulation of NF-kappaB activity through lysine monomethylation of p65. 2009. Proc Natl Acad Sci USA. 106(45):18972-7

Harder J, Franchi L, Munoz-Planillo R, Park JH, Reimer T, Nunez G. 2009. Activation of the Nlrp3 inflammasome by Streptococcus pyogenes requires streptolysin O and NF-kappa B activation but proceeds independently of TLR signaling and P2X7 receptor. J Immunol 183: 5823-9.

Kaiser WJ, Upton JW, Mocarski ES. 2008. Receptor-interacting protein homotypic interaction motif-dependent control of NF-kappa B activation via the DNA-dependent activator of IFN regulatory factors. J Immunol 181: 6427-34.

Kanda T, Yokosuka O, Nagao K, Saisho H. 2006. State of hepatitis C viral replication enhances activation of NF-kB- and AP-1-signaling induced by hepatitis B virus X.Cancer Lett 234: 143-8.

Karin M. NF-kappaB as a critical link between inflammation and cancer. 2009 Cold Spring Harb Perspect Biol. a000141. Review

Karin, M. and Greten, F. R. 2005. NF-kappaB: linking inflammation and immunity of cancer development and progression. Nat Rev Immunol. 5 (10), 749-759.

Kingeter LM, Schaefer BC. 2008. Loss of protein kinase C theta, Bcl10, or Malt1 selectively impairs proliferation and NF-kappa B activation in the CD4+ T cell subset. J Immunol 181: 6244-54.

Lim EJ, Lee SH, Lee JG, Kim JR, Yun SS, et al. 2007. Toll-like receptor 9 dependent activation of MAPK and NF-kB is required for the CpG ODN-induced matrix metalloproteinase-9 expression. Exp Mol Med 39: 239-45.

Mefert MK, Baltimore D. 2005. Physiological functions of brain NF-kappaB. Trends Neurosci. 28(1): 37-43.

Nicola JP, Nazar M, Mascanfroni ID, Pellizas CG, Masini-Repiso AM. NF-{kappa}B p65 Subunit Mediates Lipopolysaccharide-Induced Na+/I- Symporter Gene Expression by Involving Functional Interaction with the Paired Domain Transcription Factor Pax8. Mol Endocrinol.
Amount: 1 kit
Available: In stock
Listprice: €482.95
Price: €482.95
lieferbar

Get an offer

Technische Fragen zu diesem Produkt?


Produktmanager: Xenia Schulte

Email: sales@hoelzel.de

Close