Highlights |
Measure NF-kappa B activation in a wide range of mammalian species (human, mouse, rat, hamster, bovine, equine, porcine, canine) using cells or tissues. Suitable for 50 to 150 NF-kappa B activation assays based on one assay per semi-confluent 10 cm cell culture dish, or a well in a 6-well culture dish, respectively. Offers a more reliable, selective and specific quantitative analysis compared to ELISA formats. Independently validated in multiple published investigations. |
Background |
Following stimuli that elicit the NF-kB pathway, the IkB inhibitory subunit of the NF-kappa B complex is phosphorylated, ubiquitinated and degraded, exposing nuclear targeting signals in the transcription factor subunits of the NF-kappa B complex. The NF-kappa B transcription factor subunits, p65 and p50, with exposed nuclear targeting signals, then translocate into the nucleus. This NF-kappa B assay kit provides an accurate quantitative estimate of NF-kappaB activation by measuring p65 (RelA) protein that has been translocated to the nucleus. |
References |
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