Comparison

NF-kappa B, NF-kB, NF-kappaB, NF-{kappa}B, Nuclear Factor-Kappa B, Activation Assay Kit (NFKB-2)

Manufacturer Fivephoton
Category
Type Kit
Specific against other
Amount 1 kit
Item no. NFKB-2
Targets NFKB1
eClass 6.1 32161000
eClass 9.0 32161090
Available
Highlights
Measure NF-kappa B activation in a wide range of mammalian species (human, mouse, rat, hamster, bovine, equine, porcine, canine) using cells or tissues.
Suitable for 50 to 150 NF-kappa B activation assays based on one assay per semi-confluent 10 cm cell culture dish, or a well in a 6-well culture dish, respectively.
Offers a more reliable, selective and specific quantitative analysis compared to ELISA formats.
Independently validated in multiple published investigations.
Background
Following stimuli that elicit the NF-kB pathway, the IkB inhibitory subunit of the NF-kappa B complex is phosphorylated, ubiquitinated and degraded, exposing nuclear targeting signals in the transcription factor subunits of the NF-kappa B complex. The NF-kappa B transcription factor subunits, p65 and p50, with exposed nuclear targeting signals, then translocate into the nucleus. This NF-kappa B assay kit provides an accurate quantitative estimate of NF-kappaB activation by measuring p65 (RelA) protein that has been translocated to the nucleus.
References
Baltimore D. Discovering NF-kappaB. 2009 Cold Spring Harb Perspect Biol. Jul; 1(1): a000026.

Bellarosa D, Binaschi M, Maggi CA, Goso C. 2005. Sabarubicin- (MEN 10755) and paclitaxel show different kinetics in nuclear factor-kappaB (NF-kB) activation: effect of parthenolide on their cytotoxicity. Anticancer Res 25: 2119-28.

Campo GM, Avenoso A, Campo S, D'Ascola A, Traina P, Calatroni A. 2008. Chondroitin-4-sulphate inhibits NF-kB translocation and caspase activation in collagen-induced arthritis in mice. Osteoarthritis Cartilage 16: 1474-83.

Cascinu S, Berardi R, Salvagni S, Beretta GD, Catalano V, et al. 2008. A combination of gefitinib and FOLFOX-4 as first-line treatment in advanced colorectal cancer patients. A GISCAD multicentre phase II study including a biological analysis of EGFR overexpression, amplification and NF-kB activation. Br J Cancer 98: 71-6.

Chen HH, Chen TW, Lin H. 2009. Prostacyclin-induced peroxisome proliferator-activated receptor-alpha translocation attenuates NF-kappaB and TNFalpha activation after renal ischemia-reperfusion injury. Am J Physiol Renal Physiol 297: F1109-18.

Ea CK, Baltimore D. Regulation of NF-kappaB activity through lysine monomethylation of p65. 2009. Proc Natl Acad Sci USA. 106(45):18972-7

Harder J, Franchi L, Munoz-Planillo R, Park JH, Reimer T, Nunez G. 2009. Activation of the Nlrp3 inflammasome by Streptococcus pyogenes requires streptolysin O and NF-kappa B activation but proceeds independently of TLR signaling and P2X7 receptor. J Immunol 183: 5823-9.

Kaiser WJ, Upton JW, Mocarski ES. 2008. Receptor-interacting protein homotypic interaction motif-dependent control of NF-kappa B activation via the DNA-dependent activator of IFN regulatory factors. J Immunol 181: 6427-34.

Kanda T, Yokosuka O, Nagao K, Saisho H. 2006. State of hepatitis C viral replication enhances activation of NF-kB- and AP-1-signaling induced by hepatitis B virus X.Cancer Lett 234: 143-8.

Karin M. NF-kappaB as a critical link between inflammation and cancer. 2009 Cold Spring Harb Perspect Biol. a000141. Review

Karin, M. and Greten, F. R. 2005. NF-kappaB: linking inflammation and immunity of cancer development and progression. Nat Rev Immunol. 5 (10), 749-759.

Kingeter LM, Schaefer BC. 2008. Loss of protein kinase C theta, Bcl10, or Malt1 selectively impairs proliferation and NF-kappa B activation in the CD4+ T cell subset. J Immunol 181: 6244-54.

Lim EJ, Lee SH, Lee JG, Kim JR, Yun SS, et al. 2007. Toll-like receptor 9 dependent activation of MAPK and NF-kB is required for the CpG ODN-induced matrix metalloproteinase-9 expression. Exp Mol Med 39: 239-45.

Mefert MK, Baltimore D. 2005. Physiological functions of brain NF-kappaB. Trends Neurosci. 28(1): 37-43.

Nicola JP, Nazar M, Mascanfroni ID, Pellizas CG, Masini-Repiso AM. NF-{kappa}B p65 Subunit Mediates Lipopolysaccharide-Induced Na+/I- Symporter Gene Expression by Involving Functional Interaction with the Paired Domain Transcription Factor Pax8. Mol Endocrinol.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 1 kit
Available: In stock
available

Delivery expected until 5/2/2024 

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