Nuclear Protein Isolation-Translocation Kit (NPI-1)

For 300+ isolations using 6-well dishes, or 100 isolations using 10 cm dishes. This kit partitions and concentrates proteins in nuclei and cytosol in a 45 minute protocol using a microcentrifuge.

In addition to direct purchase from Fivephoton Biochemicals, this product can be purchased on fishersci.com and shipped directly from Fivephoton Biochemical to the customer; Fishersci.com part number NC0797198. The Nuclear Protein Isolation Kit is also available from additional distributors of Fivephoton Biochemicals.

Simplicity: Allows for simultaneous isolations of multiple samples.
Scalability: Suitable for 300+ isolations/extractions based on a well in a 6-well dish, or 100 isolations/extractions using semi-confluent 10 cm dishes.
Versibility: Applicalbe for cells from virtually any mammalian species, or with tissues using an initial homogenization step.
Broad Downstream Applicability: Ideal for nuclei isolation, nuclei-cytosol fractionation, nuclear protein extraction, measurement of nuclear import, EMSA, transcription factor activation and translocation assays.

Al-Hanbali M, Ali D, Bustami M, Abdel-Malek S, Al-Hanbali R, et al. 2009. Epicatechin suppresses IL-6, IL-8 and enhances IL-10 production with NF-kappaB nuclear translocation in whole blood stimulated system. Neuro Endocrinol Lett 30: 131-8.

Allen PB, Kwon YG, Nairn AC, Greengard P. 1998. Isolation and characterization of PNUTS, a putative protein phosphatase 1 nuclear targeting subunit. J Biol Chem 273: 4089-95.

Anderson LG, Meeker RB, Poulton WE, Huang DY. 2009. Brain distribution of carboxy terminus of Hsc70-interacting protein (CHIP) and its nuclear translocation in cultured cortical neurons following heat stress or oxygen-glucose deprivation. Cell Stress Chaperones.

Arminan A, Gandia C, Bartual M, Garcia-Verdugo JM, Lledo E, et al. 2009. Cardiac differentiation is driven by NKX2.5 and GATA4 nuclear translocation in tissue-specific mesenchymal stem cells. Stem Cells Dev 18: 907-18.

Azuma Y, Tabb MM, Vu L, Nomura M. 1995. Isolation of a yeast protein kinase that is activated by the protein encoded by SRP1 (Srp1p) and phosphorylates Srp1p complexed with nuclear localization signal peptides. Proc Natl Acad Sci U S A 92: 5159-63.

Brown TL, Rosen JM. 1986. Isolation and characterization of milk protein nuclear RNAs in rat mammary gland. Anal Biochem 153: 211-20.

Busch H, Hnilica LS, Chien SC, Davis JR, Taylor CW. 1962. Isolation and purification of RP2-L, a nuclear protein fraction of the Walker 256 carcinosarcoma. Cancer Res 22: 637-45.

Cao Y, Liu R, Jiang X, Lu J, Jiang J, et al. 2009. Nuclear-cytoplasmic shuttling of menin regulates nuclear translocation of {beta}-catenin. Mol Cell Biol 29: 5477-87.

G, Favini E, Degl'Innocenti D, Salvi A, De Petro G, et al. 2009. RET/PTC1-driven neoplastic transformation and proinvasive phenotype of human thyrocytes involve Met induction and beta-catenin nuclear translocation. Neoplasia 11: 10-21.

Chaitanya GV, Babu PP. 2008. Multiple apoptogenic proteins are involved in the nuclear translocation of Apoptosis Inducing Factor during transient focal cerebral ischemia in rat. Brain Res 1246: 178-90.