Nuclear Protein Isolation-Translocation Kit (NPI-1)

Manufacturer Fivephoton
Category Products / Kits
Type Kit
Specific against other
Amount 1 kit
ArtNr NPI-1
Eclass 6.1 32161000
Eclass 9.0 32161090
Available
Highlights
For 300+ isolations using 6-well dishes, or 100 isolations using 10 cm dishes. This kit partitions and concentrates proteins in nuclei and cytosol in a 45 minute protocol using a microcentrifuge.

In addition to direct purchase from Fivephoton Biochemicals, this product can be purchased on fishersci.com and shipped directly from Fivephoton Biochemical to the customer; Fishersci.com part number NC0797198. The Nuclear Protein Isolation Kit is also available from additional distributors of Fivephoton Biochemicals.

Simplicity: Allows for simultaneous isolations of multiple samples.
Scalability: Suitable for 300+ isolations/extractions based on a well in a 6-well dish, or 100 isolations/extractions using semi-confluent 10 cm dishes.
Versibility: Applicalbe for cells from virtually any mammalian species, or with tissues using an initial homogenization step.
Broad Downstream Applicability: Ideal for nuclei isolation, nuclei-cytosol fractionation, nuclear protein extraction, measurement of nuclear import, EMSA, transcription factor activation and translocation assays.
Background
A key regulatory event in transcription factor activation is the translocation process from cytosol to nucleus. Proteins with exposed targeting signals interact with the nuclear pore import complex and enter into the nucleus to perform regulatory functions. The Nuclear Protein Isolation Kit provides a convenient method to isolate nuclear proteins, and detect and quantitate cytoplasmic to nuclear import.

Downstream applications for the nuclear protein isolation kit include, but are not limited to, quantitation of transcription factor activation, EMSA, cellular localization studies, nuclear protein isolation, and general investigative mechanisms of nuclear import.

Transmembrane proteins, and other polypeptides, with nuclear import signals may become proteolytically cleaved and translocate through the nuclear pore to bind chromosomal regulatory elements. The nuclear protein isolation protocol provided with this kit can be applied to identify and quantitate the nuclear import of the generated peptide fragments.
References
Al-Hanbali M, Ali D, Bustami M, Abdel-Malek S, Al-Hanbali R, et al. 2009. Epicatechin suppresses IL-6, IL-8 and enhances IL-10 production with NF-kappaB nuclear translocation in whole blood stimulated system. Neuro Endocrinol Lett 30: 131-8.

Allen PB, Kwon YG, Nairn AC, Greengard P. 1998. Isolation and characterization of PNUTS, a putative protein phosphatase 1 nuclear targeting subunit. J Biol Chem 273: 4089-95.

Anderson LG, Meeker RB, Poulton WE, Huang DY. 2009. Brain distribution of carboxy terminus of Hsc70-interacting protein (CHIP) and its nuclear translocation in cultured cortical neurons following heat stress or oxygen-glucose deprivation. Cell Stress Chaperones.

Arminan A, Gandia C, Bartual M, Garcia-Verdugo JM, Lledo E, et al. 2009. Cardiac differentiation is driven by NKX2.5 and GATA4 nuclear translocation in tissue-specific mesenchymal stem cells. Stem Cells Dev 18: 907-18.

Azuma Y, Tabb MM, Vu L, Nomura M. 1995. Isolation of a yeast protein kinase that is activated by the protein encoded by SRP1 (Srp1p) and phosphorylates Srp1p complexed with nuclear localization signal peptides. Proc Natl Acad Sci U S A 92: 5159-63.

Brown TL, Rosen JM. 1986. Isolation and characterization of milk protein nuclear RNAs in rat mammary gland. Anal Biochem 153: 211-20.

Busch H, Hnilica LS, Chien SC, Davis JR, Taylor CW. 1962. Isolation and purification of RP2-L, a nuclear protein fraction of the Walker 256 carcinosarcoma. Cancer Res 22: 637-45.

Cao Y, Liu R, Jiang X, Lu J, Jiang J, et al. 2009. Nuclear-cytoplasmic shuttling of menin regulates nuclear translocation of {beta}-catenin. Mol Cell Biol 29: 5477-87.

G, Favini E, Degl'Innocenti D, Salvi A, De Petro G, et al. 2009. RET/PTC1-driven neoplastic transformation and proinvasive phenotype of human thyrocytes involve Met induction and beta-catenin nuclear translocation. Neoplasia 11: 10-21.

Chaitanya GV, Babu PP. 2008. Multiple apoptogenic proteins are involved in the nuclear translocation of Apoptosis Inducing Factor during transient focal cerebral ischemia in rat. Brain Res 1246: 178-90.
Amount: 1 kit
Available: In stock
Listprice: €282.05
Price: €282.05
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