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Fluorescent Dopamine Neurotransmitter Ligand (DnsylD-1)

Fluorescent Dopamine Neurotransmitter Ligand (DnsylD-1)

Item no.	DnsylD-1-5mg
Manufacturer	Fivephoton
Amount	5 mg
Category	Neuroscience Molecular Targets for Neuroscience Targets for Rodent Neurosciences Neural Signaling Reagents & Chemicals Molecules
Type	Molecules
Specific against	other
Citations	May S, Andreasson-Ochsner M, Fu Z, Low YX, Tan D, de Hoog HP, Ritz S, Nallani M, Sinner EK. 2013. In Vitro Expressed GPCR Inserted in Polymersome Membranes for Ligand-Binding Studies. Angew. Chem. Int. Ed, 52, 749 -753
ECLASS 10.1	32169090
ECLASS 11.0	32169090
UNSPSC	12000000
Available
Manufacturer - Targets	NDRG2
Storage Conditions	4°C
Description	Highlights Pharmalogical properties virtually identical to native dopamine. DnsylD-1 operates selectively in uptake and binding assays at nanomolar concentrations. The native dopamine chemical structure is the major component of this fluorescent catecholamine, generating a fluorescent neurotransmitter molecule with high affinity toward all dopamine receptor subtypes as well as to the dopamine transporter. The small dansyl tag with a 5-carbon polylinker to dopamine generates a stable, bright fluorescent signal with negligible steric hindrance. The dansyl fluorescent tag offers a large stokes shift that minimizes autofluorescence and non-specific background signals: (Ex/Em: 313/515nm). The fluorescent signal of DnsylD-1TM is blockable by standard inhibitors to dopamine receptors and DAT. Standard inhibitors can be employed to distinguish the interaction of DnsylD-1TM with dopamine receptors and DAT. Fluorescence from DnsylD-1TM colocalizes with tyrosine hydroxylase and identifies dopaminergic neurons. To assay for dopamine receptor binding, first suspend the provided solid in DMSO (10 - 50 µl). Centrifuge to clarify, and collect the DMSO soluble fraction, which can be aliquoted and stored at -20oC: DnsylD-1TM when suspended in DMSO and frozen at -20oC is stable for at least 2 years. Dilute the DMSO solubilized material into your assay buffer (for example PBS-BSA) to the desired assay concentration. Detect DnsylD-1TM by fluorescent microscopy, with a fluorescent
Background	Fluorescent Dopamine Neurotransmitter Ligand (Dansyl-Dopamine), Overview This fluorescent labeled catecholamine neurotransmitter analog of dopamine (Product Name: DnsylD-1TM), with dansyl attached by a 5-carbon spacer arm to dopamine, provides a unique pharmacological tool. DnsylD-1TM is a stable, receptor selective and high affinity pharmacological ligand that can be detected by fluorescent microscopy and FLIPR devices. Unlike recently reported synthesized fluorescent neurotransmitter analogs, DnsylD-1TM contains the entire native dopamine structural moiety attached with the 5-carbon polylinker to the small fluorescence label dansyl. This structural modification minimizes steric hindrance, making DnsylD-1TM an effective reagent for dopamine ligand binding and transporter uptake assays at namomolar concentrations. A large stokes shift between excitation and emission maxima (Ex:Em, 313:515 nm) of DnsylD-1TM provides high signal to noise detection with minimal autofluorescence. The dansyl moiety also changes spectral characteristics on intermolecular contact, facilitating biophysical characterization of ligand-receptor interaction. DnsylD-1TM is a suitable substitute for radioactive ligand. Applications for Fluorescent Dopamine Ligand-Neurotransmitter (DnsylD-1TM) DnsylD-1TM has the following applicability due to the small molecular size of the dansyl fluorescent label, a long spacer arm between the dopamine structure and the fluorescent tag, stability in aqueous solution and low autofluorescence of the surrounding cellular or tissue medium. Histochemical imaging and quantitation of dopamine receptor expression in vivo and vitro. Pharmacological applications as a substitute for radioactive ligand. Dopamine metabolism and turnover analysis. Dopamine uptake analysis. Dopaminergic neuron detection. Analysis of disease conditions associated with altered dopamine synthesis, dopamine receptor expression or dopamine uptake: human disease research applicability in the following: a) Parkinson's disease. b) Psychological disorder, including ADHD, schizophrenia and substance abuse. c) Physiological regulation of cardiovascular, renal and immune function.
Highlights	Dansyl labeled dopamine that operates selectively in binding and uptake assays at nanomolar concentrations. The native dopamine chemical structure is the major component of this fluorescent labeled catecholamine, which generates a fluorescent neurotransmitter molecule with high affinity toward all dopamine receptor subtypes, as well as to the dopamine transporter. The small dansyl tag with a 5-carbon polylinker to dopamine generates a stable, bright fluorescent signal with negligible steric hindrance. The dansyl fluorescent tag creates a large stokes shift minimizing autofluorescence and non-specific background signal. The fluorescent signal of DnsylD-1TM is blockable by standard inhibitors to dopamine receptors and DAT. Standard inhibitors can also be employed to distinguish the interaction of DnsylD-1TM with dopamine receptors and DAT. Fluorescence from DnsylD-1TM colocalizes with tyrosine hydroxylase thereby identifying dopaminergic neurons. To assay for dopamine receptor binding, first suspend the provided solid in DMSO (10 - 50 ul). Centrifuge and then dilute an aliquot of the supernatant into your assay buffer (for example PBS-BSA) to the desired assay concentration. DnsylD-1TM when suspended in DMSO and frozen at -20C is stable for at least 2 years. Detect DnsylD-1TM by fluorescent microscopy, with a fluorescent plate reader or by flow cytometry. Set the fluorescent detection device to the following parameters: Ex/Em: 333/515 nm.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 5 mg

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Delivery expected until 10/2/2025

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