Ferrocene NHS Ester (Amine Reactive Electrochemical Reporter: No. HPT1002) - 50 mg

Highlights:  Highly reactive NHS ester modification reagent to primary amines generating high yields of ferrocene conjugates. Widely employed in commercial oligonucleotide synthesis due to its efficacy. Since typically conjugated to primary amines with poly-C extensions, steric hindrance is negligible. Efficiently conjugated to primary amines on peptides, proteins and modified oligonucleotides

Ferrocene-NHS Ester is an electrochemical active reporter molecule that reacts with primary amines in peptides, proteins and oligonucleotides. The NHS reactive group allows for conjugation to both alpha and epsilon amino groups in peptide chains, and to free thiol and amines in modified nucleotides. Conjugation of ferrocene-NHS ester to the epsilon-amino group in lysine provides a multi-carbon linker between the polypeptide backbone and the ferrocene reporter, which limits steric hindrance on intermolecular interactions. Below is a schematic diagram revealing the reaction between Ferrocene-NHS ester and the epsilon-amino group in lysine, outlining the method of conjugation to peptide chains. A similar scheme applies toward conjugating to modified oligonucleotides. Conjugated ferrocene is detected with voltammetry.