Transmembrane Protein Extraction Reagent (Part No. tmPER-50)

Manufacturer Fivephoton
Category Products / Proteins
Type Proteins
Specific against other
Amount 30 ml
ArtNr TmPER-50
Eclass 6.1 34160400
Eclass 9.0 42020190
Available
Highlights
Extracts approximately 90% of proteins from tissues, including transmembrane proteins.
Simple and rapid protocol for tissue protein extraction. Use mini-homogenizer-pestle, microcentrifuge tubes and table top microcentrifuge.
Maintains protein-protein complexes for downstream immunoprecipitation and pull-down experiments. ELISA compatible. Non-denaturing.
Can be applied directly to extract tissue proteins for Western blotting.
Compatible with Bradford assay.
Absence of amines in the buffer allows for NHS-Ester conjugation and biotinylation.
Background
Protocol (Keep solutions on ice):
Pulverize approximately 90 uL of tissue. Place tissue in a 1.5 ml round bottom microcentrifuge tube.
Add general phosphatase and protease inhibitor cocktails to 500 uL of ice-cold Tissue Protein Extraction Buffer.
Add 500 uL Tissue Protein Extraction buffer with inhibitors to pulverized tissue.
Homogenize tissue with a mini pestle-homogenizer using 15 strokes, 3 seconds/stroke on ice.
Centrifuge 12000g for 15 min at 4C.
Remove supernatant (without lipid layer) and transfer into another 1.5 ml tube.
Centrifuge again at 12000g for 15 min at 4C.
Transfer supernatant to another tube. The supernatant fraction contains the extracted proteins.
The Bradford assay can be used to quantitate extracted protein concentration.
Amount: 30 ml
Available: In stock
Listprice: €121.78
Price: €121.78
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