BACE1 Antibody (26-45), pAb, Rabbit

USA

GenScript Rabbit Anti-BACE1 (26-45) Polyclonal Antibody can specifically detect human BACE1. Sequence homology predicts that it will also react with mouse, rat, and bovine BACE1.

beta-secretase, also called BACE (beta-site of APP cleaving enzyme), is an aspartic-acid protease important in the pathogenesis of Alzheimers disease, and in the formation of myelin sheaths in peripheral nerve cells. BACE1, also known as memapsin-2, is the major beta-secretase for generation of the 40 or 42 amino-acid-long beta-amyloid peptides that aggregate in the brain of Alzheimers patients. Extracellular cleavage of APP by BACE1 releases a soluble extracellular fragment and is followed by APP cleavage within its transmembrane domain by gamma-secretase. The second cleavage releases the intracellular domain of APP and beta-amyloid. The immature form of BACE1 contains an N-terminal signal sequence (residues 1–21) followed by a large catalytic domain, a single transmembrane domain (residues 461–477), and a short cytoplasmic domain (residues 478–501). The signal sequence (1-21) is cleaved from the immature form, yielding the proBACE1 protein, which starts at residue 22. The proBACE1 protein is modified by cleavage of 24 N-terminal residues (aa 22-45), producing the mature BACE1 protein. GenScript, Rabbit Anti-BACE1 (26-45) Polyclonal Antibody, is developed in rabbit using a synthetic peptide, IRLPLRSGLGGAPLGLRLPR, corresponding to amino acids 26-45 at the N-terminal of human BACE1. GenScript Rabbit Anti-BACE1 (26-45) pAb is highly purified from rabbit antiserum by immunoaffinity chromatography and is supplied as 40 ug aliquots at concentration of 0.5 mg/ml.

Immunoaffinity chromatography