PKCu Antibody, pAb, Rabbit

USA

GenScript Rabbit Anti-PKCμ, Polyclonal Antibody detects endogenous levels of human and mouse PKCμ, protein. No cross-reactivity to other PKC isoforms is detected.

Protein kinase C (PKC, EC 2.7.11.13) is a cyclic, nucleotide-independent enzyme that phosphorylates serine and threonine residues. The PKC family is divided into three groups depending on each the enzymes cofactor requirements: conventional (c)PKC isoforms (alpha, betaI, betaII, and gamma) require diacylglycerol (DAG), Ca2+, and phospholipids for activation, novel (n)PKC isoforms (delta, epsilon, eta (also known as PKC-L), theta, and u the mouse homolog of human PKCu (also known as PKD)) require DAG but not Ca2+ for activation, and atypical (a)PKC isoforms, (zeta, iota, and lambda, the mouse homolog of human PKC iota) require neither Ca2+ nor DAG. A new PKC member has recently been discovered and is referred to as PKCny. PKCs phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. These kinases also serve as major receptors for phorbol esters, a class of tumor promoters. Every member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. PKCu, also known as nPKC-D1 and nPKC-mu, is a member of PKC family of serine/threonine kinases. PKCu has two characteristic cysteine-rich repeats, a conserved ATP-binding consensus sequence in the kinase domain, and the invariate aspartate essential for kinase activity. GenScript, Rabbit Anti-PKCu Polyclonal Antibody, is developed in rabbit using a KLH-coupled synthetic peptide corresponding to residues of human PKCu protein.

Immunoaffinity chromatography