Akt Antibody (Ser473), pAb, Rabbit

USA

Rabbit Anti-Akt (Ser473) Polyclonal Antibody detects endogenous human and mouse total Aktα, Aktβ, and Aktγ, proteins. It has not yet been tested with other species.

Akt, also known as protein kinase B (PKB), a serine/ threonine kinase, is a critical enzyme in several signal transduction pathways involved in cell proliferation, apoptosis, angiogenesis, and diabetes. Akt is activated following its phosphorylation at two regulatory residues. Phosphorylation of threonine on the kinase domain, catalyzed by PDK1, is essential for Akt activation. Akt activity is augmented approximately 10-fold by phosphorylation at the serine on the hydrophobic motif by PDK2. Phosphorylation of Thr308 and Ser473 activates Akt alpha. Phosphorylation at Thr309 and Ser474 on Akt beta1 and beta2, and on Thr305 on Akt gamma result in their activation. Akt promotes cell survival by inhibiting apoptosis by phosphorylating and inactivating several targets, including Bad, forkhead transcription factors, c-Raf and caspase-9. The activation of Akt is negatively regulated by PTEN, a PIP3 specific phosphatase, and SHIP, a SH2-domain containing inositol 5-phosphatase. GenScript, Rabbit Anti-Akt (Ser473) Polyclonal Antibody, is developed in rabbit using a synthetic peptide corresponding to residues surrounding S473 of human, rat and mouse Akt alpha, also known as protein kinase B alpha (PKBalpha), Akt1, and RACalpha. In addition, this peptide is identical to the sequence surrounding S474 of human, rat and mouse Akt beta (Akt2) and S472 of human and mouse Akt gamma (Akt3).

Immunoaffinity chromatography

57 kD

Small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. Before use, briefly centrifuge the vial centrifuge to dislodge any liquid in the container's cap.