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Interleukin-6 (IL-6), Human

Interleukin-6 (IL-6), Human

Item no.	Z00372-1
Manufacturer	GenScript
Amount	1mg(2x500ug)
Category	Oncology Food Safety Neuroscience Neural Lineage Markers Metabolism Diabetes Glucose Homeostasis Neural Transcription Factors By Cell Type Carcinoma Hematology Leukemia Peptides & Proteins Recombinant Proteins Cell Biology Cell Movement Cell Adhesion Obesity Cell Culture Cytokines Cell Status Necrosis Contaminants Metals By Organ Breast Cancer Heart & Blood Cancer Bone Cancer
Type	Proteins
Specific against	Human
Purity	Greater than 98.0% as determined by: (a) Analysis by RP-HPLC (b) Anion-exchange FPLC (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel
Sequence	PVPPGEDSKD VAAPHRQPLT SSERIDKQIR YILDGISALR KETCNKSNMC ESSKEALAEN NLNLPKMAEK DGCFQSGFNE ETCLVKIITG LLEFEVYLEY LQNRFESSEE QARAVQMSTK VLIQFLQKKA KNLDAITTPD PTTNASLLTK LQAQNQWLQD MTTHLILRSF KEFLQSSLRA LRQM
Citations	Wernstedt I, et al . Reduced stress- and cold-induced increase in energy expenditure in IL-6 deficient mice. Am. J. Physiol. Regul. Integr. Comp. Physiol.Feb 2006, Itoh S, et al . A critical role for interleukin-6 family-mediated Stat3 activation in osteoblast differentiation and bone formation. Bone. Sep 2006, 39 (3): 505-512. Pang Y, et al . Role of interleukin-6 in lipopolysaccharide-induced brain injury and behavioral dysfunction in neonatal rats. Neuroscience. Aug 2006, 141 (2): 745-755.
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	protein/Z00372_1-Interleukin_6_IL_6_Human,Interleukin-6 (IL-6), is a multifunctional protein originally discovered in the media of cells stimulated with double stranded RNA. Interleukin-6 (IL-6) induces multiple effects, as indicated by its numerous synonyms: plasmacytoma growth factor, interferon-beta-2, monocyte derived human B cell growth factor, B cell stimulating factor, hepatocyte stimulating factor, Interleukin Hybridoma/Plasmacytoma-1. Recombinant human Interleukin-6 (IL-6), that is produced in, E. coli, and purified by proprietary chromatographic techniques is a single, non-glycosylated polypeptide chain containing 185 amino acids and having a molecular mass of 21,000 Da.</td></tr><tr><th>M.W.</th><td colspan="7"> 21,000 Da</td></tr><tr><th>Purity</th><td colspan="7"> Greater than 98.0% as determined by: (a) Analysis by RP-HPLC (b) Anion-exchange FPLC (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel</td></tr><tr><th>Endotoxin Level</th><td colspan="7"> Less than 0.1ng/ug (1 EU/ug) determined by LAL test.</td></tr><tr><th>Specific Activity</th><td colspan="7"> The ED50 as determined by the dose-dependant stimulation of murine 7TD1 cells is < 0.1 ng/ml, corresponding to a Specific Activity of 5×107 IU/mg. </td></tr><tr><th>Storage</th><td colspan="7"> Lyophilized, recombinant human Interleukin-6 (IL-6), remains stable at room temperature for three weeks, but it is best stored desiccated below -18C. Upon reconstitution recombinant human Interleukin-6 (IL-6) should be stored at 4C for up to seven days. For long term storage it is recommended that a carrier protein (0.1% HSA or BSA) be added. Avoid repeated freeze-thaw cycles.</td></tr><tr><th>Formulation</th><td colspan="7"> The protein was lyophilized after extensive dialysis against 20mM HAc-NaAc, pH5.0, 150mM NaAc buffer.</td></tr><tr><th>Reconstitution</th><td colspan="7"> It is recommended to reconstitute the lyophilized recombinant human Interleukin-6 (IL-6) in sterile 18 Momega-cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.</td></tr><tr><th>Sequence</th><td colspan="7"> PVPPGEDSKD VAAPHRQPLT SSERIDKQIR YILDGISALR KETCNKSNMC ESSKEALAEN NLNLPKMAEK DGCFQSGFNE ETCLVKIITG LLEFEVYLEY LQNRFESSEE QARAVQMSTK VLIQFLQKKA KNLDAITTPD PTTNASLLTK LQAQNQWLQD MTTHLILRSF KEFLQSSLRA LRQM </td></tr><tr><th>Reference</th><td colspan="7">Wernstedt I, et al . Reduced stress- and cold-induced increase in energy expenditure in IL-6 deficient mice. Am. J. Physiol. Regul. Integr. Comp. Physiol.Feb 2006, Itoh S, et al . A critical role for interleukin-6 family-mediated Stat3 activation in osteoblast differentiation and bone formation. Bone. Sep 2006, 39 (3): 505-512. Pang Y, et al . Role of interleukin-6 in lipopolysaccharide-induced brain injury and behavioral dysfunction in neonatal rats. Neuroscience. Aug 2006, 141 (2): 745-755.</td></tr>
Similar products	Interleukin-6
Available
Specificity	The ED50 as determined by the dose-dependant stimulation of murine 7TD1 cells is < 0.1 ng/ml, corresponding to a Specific Activity of 5x107 IU/mg.
Specificity	The ED50 as determined by the dose-dependant stimulation of murine 7TD1 cells is < 0.1 ng/ml, corresponding to a Specific Activity of 5x107 IU/mg.
Description	Interleukin-6 (IL-6), is a multifunctional protein originally discovered in the media of cells stimulated with double stranded RNA. Interleukin-6 (IL-6) induces multiple effects, as indicated by its numerous synonyms: plasmacytoma growth factor, interferon-beta-2, monocyte derived human B cell growth factor, B cell stimulating factor, hepatocyte stimulating factor, Interleukin Hybridoma/Plasmacytoma-1. Recombinant human Interleukin-6 (IL-6), that is produced in, E. coli, and purified by proprietary chromatographic techniques is a single, non-glycosylated polypeptide chain containing 185 amino acids and having a molecular mass of 21, 000 Da.
Endotoxin Level	Less than 0.1ng/ug (1 EU/ug) determined by LAL test.
Formulation	The protein was lyophilized after extensive dialysis against 20mM HAc-NaAc, pH5.0, 150mM NaAc buffer.
M.W.	21, 000 Da
Product Line	Cytokine, Chemokines & Growth Factors
Reconstitution	It is recommended to reconstitute the lyophilized recombinant human Interleukin-6 (IL-6) in sterile 18 Momega-cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
Storage	Lyophilized, recombinant human Interleukin-6 (IL-6), remains stable at room temperature for three weeks, but it is best stored desiccated below -18C. Upon reconstitution recombinant human Interleukin-6 (IL-6) should be stored at 4C for up to seven days. For long term storage it is recommended that a carrier protein (0.1% HSA or BSA) be added. Avoid repeated freeze-thaw cycles.
Product Origin	USA

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 1mg(2x500ug)

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