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Stem Cell Factor (SCF), Human

Stem Cell Factor (SCF), Human

Item no.	Z00400-1
Manufacturer	GenScript
Amount	1 mg (2x500 ug)
Category	Oncology Food Safety Peptides & Proteins Recombinant Proteins Cell Biology Cell Movement Cell Adhesion Contaminants Metals By Organ Breast Cancer Heart & Blood Cancer Bone Cancer
Type	Proteins
Specific against	Human (Homo sapiens)
Purity	Greater than 95.0% as determined by the following methods: (a) Analysis by SEC-HPLC (b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel
Citations	Lili W, et al . Expression, Renaturation and Simultaneous Purification of Recombinant Human Stem Cell Factor in Escherichia coli. Biotechnol. Lett. Jul 2006, 28(13): 993-997. Baccarani U, et al . Implication of stem cell factor in human liver regeneration after transplantation and resection. Growth Factors. Jun 2006, 24(2): 107-110. Da Silva CA, et al . Effect of glucocorticoids on stem cell factor expression in human asthmatic bronchi. Clin. Exp. Allergy. Mar 2006, 36(3): 317-324.
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	protein/Z00400-Stem_Cell_Factor_SCF_human,Human Stem Cell Factor (SCF) , produced in, E. coli, is a single, non-glycosylated polypeptide chain containing 165 amino acids and having a molecular mass of 18,409 Da. Stem Cell Factor (SCF), is a hematopoietic growth factor that exerts its activity during the early stages of hematopoiesis. Stem Cell Factor (SCF) stimulates the proliferation of myeloid, erythroid, and lymphoid progenitors in bone marrow cultures and has been shown to act synergistically with colony stimulating factors.</td></tr><tr><th>M.W.</th><td colspan="7"> 18,409 Da</td></tr><tr><th>Purity</th><td colspan="7"> Greater than 95.0% as determined by the following methods: (a) Analysis by SEC-HPLC (b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel</td></tr><tr><th>Endotoxin Level</th><td colspan="7"> Less than 0.1 ng/ug (IEU/ug) of rHuSCF</td></tr><tr><th>Specific Activity</th><td colspan="7"> The ED50, as determined by the dose-dependant stimulation of human TF-1 cells, is < 2 ng/ml, corresponding to a specific activity of 5×105 IU/mg. </td></tr><tr><th>Storage</th><td colspan="7"> Lyophilized, recombinant human stem cell factor (SCF), remains stable at room temperature for three weeks, but it is best stored desiccated below -18C. Upon reconstitution Recombinant Human Stem Cell Factor (SCF) should be stored at 4C for up to seven days. For long term storage it is recommended that a carrier protein (0.1% HSA or BSA ) be added. Avoid repeated freeze-thaw cycles.</td></tr><tr><th>Formulation</th><td colspan="7"> The protein was lyophilized after extensive dialysis against 20mM HAc-NaAc, pH4.5, 200mM NaCl buffer.</td></tr><tr><th>Reconstitution</th><td colspan="7"> It is recommended that the lyophilized, recombinant human stem cell factor (SCF), be reconstituted in sterile 18 Momega-cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.</td></tr><tr><th>Reference</th><td colspan="7">Lili W, et al . Expression, Renaturation and Simultaneous Purification of Recombinant Human Stem Cell Factor in Escherichia coli. Biotechnol. Lett. Jul 2006, 28(13): 993-997. Baccarani U, et al . Implication of stem cell factor in human liver regeneration after transplantation and resection. Growth Factors. Jun 2006, 24(2): 107-110. Da Silva CA, et al . Effect of glucocorticoids on stem cell factor expression in human asthmatic bronchi. Clin. Exp. Allergy. Mar 2006, 36(3): 317-324.</td></tr>
Similar products	Stem
Available
Specificity	The ED50, as determined by the dose-dependant stimulation of human TF-1 cells, is < 2 ng/ml, corresponding to a specific activity of 5x105 IU/mg.
Country of Origin	USA
Storage Conditions	Lyophilized, recombinant human stem cell factor (SCF), remains stable at room temperature for three weeks, but it is best stored desiccated below -18C. Upon reconstitution Recombinant Human Stem Cell Factor (SCF) should be stored at 4C for up to seven days. For long term storage it is recommended that a carrier protein (0.1% HSA or BSA ) be added. Avoid repeated freeze-thaw cycles.
Molecular Weight	18, 409 Da
Product Line	Cytokine, Chemokines & Growth Factors
Manufacturer - Specificity	The ED50, as determined by the dose-dependant stimulation of human TF-1 cells, is < 2 ng/ml, corresponding to a specific activity of 5x105 IU/mg.
Description	Human Stem Cell Factor (SCF) , produced in, E. coli, is a single, non-glycosylated polypeptide chain containing 165 amino acids and having a molecular mass of 18, 409 Da. Stem Cell Factor (SCF), is a hematopoietic growth factor that exerts its activity during the early stages of hematopoiesis. Stem Cell Factor (SCF) stimulates the proliferation of myeloid, erythroid, and lymphoid progenitors in bone marrow cultures and has been shown to act synergistically with colony stimulating factors.
Reconstitution	It is recommended that the lyophilized, recombinant human stem cell factor (SCF), be reconstituted in sterile 18 Momega-cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
Formulation	The protein was lyophilized after extensive dialysis against 20mM HAc-NaAc, pH4.5, 200mM NaCl buffer.
Endotoxin Level	Less than 0.1 ng/ug (IEU/ug) of rHuSCF

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Z00400-1-datasheet.pdf

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