« Back
Home /
Anti-Apolipoprotein A-II (Human)

Anti-Apolipoprotein A-II (Human)

Item no.	18-732-292279
Manufacturer	GENWAY
Amount	1 mg
Category	Metabolism Glucose Homeostasis Cholesterol & Lipid Metabolism Antibodies Obesity Immunohistochemistry Immunoprecipitation Western Blot ELISA Lipid & Fatty Acid Transport Primary Antibodies
Type	Antibody
Applications	WB, IP, IHC, ELISA
Specific against	Human (Homo sapiens)
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	GWB-A197DF
Similar products	18-732-292279
Available
Genway ID:	GWB-A197DF
Antigen:	apoLipoprotein A2
Immunogen:	apoLipoprotein Type A-II was isolated from human plasma by density gradient centrifugation followed by HPLC purification. Family: ApolipoproteinFraction: Affinity Purified IgGApplication: Anti-apoLipoprotein antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve for immunoprecipitation and for western blotting for highly sensitive qualitative analysis. Recommended
Dilution:	This product was assayed by immunoblot and found to be reactive against apoLipoprotein A-II at a dilution of 1:5 000 to 1:10 000. This product was also assayed against 1. 0 ?g of apoLipoprotein A-II in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H& L] (Rabbit) catalog # 25-732-294061 and (ABTS (2 2\' -azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:4 000 to 1:8 000 of the stock concentration is suggested for this product. For immunohistochemistry on paraffin embedded tissue dilute the product 1:50 to 1:200. Optimal titers for other applications should be determined by the researcher. Physical State: Liquid (sterile filtered)Purity and
Specificity:	This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other apoLipoproteins and human serum proteins to remove any unwanted specificities. Typically less than 1% cross reactivity against other types of apoLipoprotein was detected by ELISA against purified standards. This antibody reacts with human apoLipoprotein A-II and has negligible cross-reactivity with Type A-I B C-I C-II C-III E and J apoLipoproteins. Specific cross reaction of anti-apoLipoprotein antibodies with antigens from other species has not been determined. Non-specific cross reaction of anti-apoLipoprotein antibodies with other human serum proteins is negligible. Antibody
Concentration:	1. 0 mg/ml (by UV absorbance at 280 nm)
Buffer:	0. 125M Sodium Borate 0. 075M Sodium Chloride 0. 005M EDTA; pH 8. 0
Stabilizer:	None
Ship Code:	WETICE
Function:	May stabilize HDL (high density lipoprotein) structure by its association with lipids and affect the HDL metabolism.
Subunit:	Homodimer; disulfide-linked. Also forms a disulfide-linked heterodimer with APOD. Interacts with HCV core protein. Interacts with APOA1BP.
Subcellular Location:	Secreted.
Tissue Specificity:	Plasma; synthesized in the liver and intestine. Mass Spectrometry: Mass=8701. 2; Method=MALDI; Range=24-100; Source=PubMed:12562854; . Mass Spectrometry: Mass=8823. 4; Method=MALDI; Range=24-100; Note=Cysteinylated ApoA-II; Source=PubMed:12562854; . Mass Spectrometry: Mass=17421. 3; Method=MALDI; Range=24-100; Note=Homodimer; Source=PubMed:12562854; . Mass Spectrometry: Mass=17293. 4; Method=MALDI; Range=24-100; Note=Heterodimer with apolipoprotein A-II(1-76); Source=PubMed:12562854; . Mass Spectrometry: Mass=8578. 3; Method=MALDI; Range=24-99; Source=PubMed:12562854; . Mass Spectrometry: Mass=17166. 2; Method=MALDI; Range=24-99; Note=Homodimer; Source=PubMed:12562854; .
Similarity:	Belongs to the apolipoprotein A2 family.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

Request product datasheet

Request safety datasheet

Simple Order Order Subscription

Amount: 1 mg

available

Delivery expected until 8/7/2025

Express Delivery: Save 8 days

Compare

Add to wishlist

Get an offer