MOUSE ANTI HUMAN FACTOR VIII

FACTOR VIII

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Recognises human Factor VIII an essential blood coagulation factor. Whilst circulating in the blood it is mostly stably complexed to von Willebrand factor. It is activated through cleavage at various sites dissociates from the complex and interacts with Factor IXa in the presence of calcium ions and phospholipids to convert Factor X to the activated Factor Xa which activates thrombin. Thrombin cleaves fibrinogen into fibrin which polymerises and cross-links to form a blood clot. The activated Factor VIII is proteolytically inactivated and cleared from the bloodstream. Defects in Factor VIII cause haemophilia A a disorder characterised by the body\' s inability to control blood clotting. This could result in severe blood loss even with minor injuries. Clone RFF-VIIIC/5 recognises an epitope towards the C-terminus of full-length Factor VIII. It also recognises the 80kDa and 70kDa cleavage products. Recommended Secondary Antibodies: Rabbit Anti Mouse IgGGoat Anti Mouse IgGGoat Anti Mouse IgG (H/L)Goat Anti Mouse IgG IgA IgMHuCAL Anti Mouse IgG1Goat Anti Mouse IgG (Fc)Sheep Anti Mouse IgG (H/L) 1. Siegel D. et al. (1998) Immunohistochemical detection of NAD(P)H:quinone oxidoreductase in human lung and lung tumors. Clin. Cancer Res. 4: 2065-2070. 2. Anwar A. et al. (2002) Interaction of the molecular chaperone Hsp70 with human NAD(P)H:quinone oxidoreductase 1. J. Biol. Chem. 277: 14060-14067. [1] \" Human dioxin-inducible cytosolic NAD(P)H:menadione oxidoreductase. cDNA sequence and localization of gene to chromosome 16. \" Jaiswal A. K. McBride O. W. Adesnik M. Nebert D. W. J. Biol. Chem. 263:13572-13578(1988) [PubMed: 2843525] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver. [2] \" Human NAD(P)H:quinone oxidoreductase (NQO1) gene structure and induction by dioxin. \" Jaiswal A. K. Biochemistry 30:10647-10653(1991) [PubMed: 1657151] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. [3] NIEHS SNPs programSubmitted (APR-2003) to the EMBL/GenBank/DDBJ databasesCited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] VARIANT SER-187. [4] \" Complete sequencing and characterization of 21 243 full-length human cDNAs. \" Ota T. Suzuki Y. Nishikawa T. Otsuki T. Sugiyama T. Irie R. Wakamatsu A. Hayashi K. Sato H. Nagai K. Kimura K. Makita H. Sekine M. Obayashi M. Nishi T. Shibahara T. Tanaka T. Ishii S. Yamamoto J. Saito K. Kawai Y. Isono Y. Nakamura Y. Nagahari K. Murakami K. Yasuda T. Iwayanagi T. Wagatsuma M. Shiratori A. Sudo H. Hosoiri T. Kaku Y. Kodaira H. Kondo H. Sugawara M. Takahashi M. Kanda K. Yokoi T. Furuya T. Kikkawa E. Omura Y. Abe K. Kamihara K. Katsuta N. Sato K. Tanikawa M. Yamazaki M. Ninomiya K. Ishibashi T. Yamashita H. Murakawa K. Fujimori K. Tanai H. Kimata M. Watanabe M. Hiraoka S. Chiba Y. Ishida S. Ono Y. Takiguchi S. Watanabe S. Yosida M. Hotuta T. Kusano J. Kanehori K. Takahashi-Fujii A. Hara H. Tanase T. -O. Nomura Y. Togiya S. Komai F. Hara R. Takeuchi K. Arita M. Imose N. Musashino K. Yuuki H. Oshima A. Sasaki N. Aotsuka S. Yoshikawa Y. Matsunawa H. Ichihara T. Shiohata N. Sano S. Moriya S. Momiyama H. Satoh N. Takami S. Terashima Y. Suzuki O. Nakagawa S. Senoh A. Mizoguchi H. Goto Y. Shimizu F. Wakebe H. Hishigaki H. Watanabe T. Sugiyama A. Takemoto M. Kawakami B. Yamazaki M. Watanabe K. Kumagai A. Itakura S. Fukuzumi Y. Fujimori Y. Komiyama M. Tashiro H. Tanigami A. Fujiwara T. Ono T. Yamada K. Fujii Y. Ozaki K. Hirao M. Ohmori Y. Kawabata A. Hikiji T. Kobatake N. Inagaki H. Ikema Y. Okamoto S. Okitani R. Kawakami T. Noguchi S. Itoh T. Shigeta K. Senba T. Matsumura K. Nakajima Y. Mizuno T. Morinaga M. Sasaki M. Togashi T. Oyama M. Hata H. Watanabe M. Komatsu T. Mizushima-Sugano J. Satoh T. Shirai Y. Takahashi Y. Nakagawa K. Okumura K. Nagase T. Nomura N. Kikuchi H. Masuho Y. Yamashita R. Nakai K. Yada T. Nakamura Y. Ohara O. Isogai T. Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Amygdala. [5] Mural R. J. Istrail S. Sutton G. G. Florea L. Halpern A. L. Mobarry C. M. Lippert R. Walenz B. Shatkay H. Dew I. Miller J. R. Flanigan M. J. Edwards N. J. Bolanos R. Fasulo D. Halldorsson B. V. Hannenhalli S. Turner R. Yooseph S. Lu F. Nusskern D. R. Shue B. C. Zheng X. H. Zhong F. Delcher A. L. Huson D. H. Kravitz S. A. Mouchard L. Reinert K. Remington K. A. Clark A. G. Waterman M. S. Eichler E. E. Adams M. D. Hunkapiller M. W. Myers E. W. Venter J. C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databasesCited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. [6] \" The status quality and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). \" The MGC Project TeamGenome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Colon. [7] Colinge J. Superti-Furga G. Bennett K. L. Submitted (OCT-2008) to UniProtKBCited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. [8] \" Lysine acetylation targets protein complexes and co-regulates major cellular functions. \" Choudhary C. Kumar C. Gnad F. Nielsen M. L. Rehman M. Walther T. Olsen J. V. Mann M. Science 325:834-840(2009) [PubMed: 19608861] [Abstract]Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-59 AND LYS-262 MASS SPECTROMETRY. [9] \" Crystal structure of human DT-diaphorase: a model for interaction with the cytotoxic prodrug 5-(aziridin-1-yl)-2 4-dinitrobenzamide (CB1954). \" Skelly J. V. Sanderson M. R. Suter D. A. Baumann U. Read M. A. Gregory D. S. J. Bennett M. Hobbs S. M. Neidle S. J. Med. Chem. 42:4325-4330(1999) [PubMed: 10543876] [Abstract]Cited for: X-RAY CRYSTALLOGRAPHY (2. 3 ANGSTROMS) OF 4-274 IN COMPLEX WITH FAD SUBUNIT. [10] \" Structures of recombinant human and mouse NAD(P)H:quinone oxidoreductases: species comparison and structural changes with substrate binding and release. \" Faig M. Bianchet M. A. Talalay P. Chen S. Winski S. Ross D. Amzel L. M. Proc. Natl. Acad. Sci. U. S. A. 97:3177-3182(2000) [PubMed: 10706635] [Abstract]Cited for: X-RAY CRYSTALLOGRAPHY (1. 7 ANGSTROMS) IN COMPLEX WITH FAD AND DUROQUINONE.