MOUSE ANTI HUMAN S100 PROTEIN

S100

Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant

Recognises S100 protein derived from human brain tissue. It is a 21kDa acidic calcium-binding protein synthesised by astroglial cells. It can be found predominantly as two isoforms the alpha-beta heterodimer (S100a) and the beta-beta homodimer (S100b). The S100 family of proteins have been implicated in a large variety of intracellular and extracellular regulatory activities such as cellular energy metabolism cytoskeleton modification cell proliferation and differentiation. When glial cells are damaged S100 protein is leaked into the extracellular matrix and cerebrospinal fluid further releasing into the bloodstream. As such S100 protein can be used as a sensitive and reliable marker for nervous system damage such as after brain damage or acute stroke.

Is sensitive to EDTA and other bivalent-binding agents. Better performance can be obtained in the presence of 5mM CaCl2 in assay buffers. Recommended Secondary Antibodies: Rabbit Anti Mouse IgGGoat Anti Mouse IgGGoat Anti Mouse IgG (H/L)Goat Anti Mouse IgG IgA IgMHuCAL Anti Mouse IgG1Goat Anti Mouse IgG (Fc)Sheep Anti Mouse IgG (H/L) [1] \" Amino acid and cDNA sequences of a vascular endothelial cell mitogen that is homologous to platelet-derived growth factor. \" Conn G. Bayne M. L. Soderman D. D. Kwok P. W. Sullivan K. A. Palisi T. M. Hope D. A. Thomas K. A. Proc. Natl. Acad. Sci. U. S. A. 87:2628-2633(1990) [PubMed: 2320579] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM VEGF-A164) PROTEIN SEQUENCE OF 27-190. [2] \" Developmental expression of vascular endothelial growth factor in the masseter muscle of rats. \" Ishii H. Oota I. Takuma T. Inomata K. Arch. Oral Biol. 46:77-82(2001) [PubMed: 11163598] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS VEGF-A188; VEGF-A164; VEGF-A144 AND VEGF-A120). [3] \" Cloning of multiple VEGF splice variants from hypoxic neonatal rat cardiomyocytes. \" Marion S. Lee T. -C. Submitted (APR-2001) to the EMBL/GenBank/DDBJ databasesCited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS VEGF-A164 AND VEGF-A188). Strain: Sprague-Dawley. [4] \" Purification and characterization of a naturally occurring vascular endothelial growth factor. placenta growth factor heterodimer. \" DiSalvo J. Bayne M. L. Conn G. Kwok P. W. Trivedi P. G. Soderman D. D. Palisi T. M. Sullivan K. A. Thomas K. A. J. Biol. Chem. 270:7717-7723(1995) [PubMed: 7706320] [Abstract]Cited for: PROTEIN SEQUENCE OF 27-40 HETERODIMERIZATION WITH PGF. Tissue: Glial tumor. [5] \" Regulation of VEGF and VEGF receptor expression in the rodent mammary gland during pregnancy lactation and involution. \" Pepper M. S. Baetens D. Mandriota S. J. Di Sanza C. Oikemus S. Lane T. F. Soriano J. V. Montesano R. Iruela-Arispe M. L. Dev. Dyn. 218:507-524(2000) [PubMed: 10878616] [Abstract]Cited for: FUNCTION TISSUE SPECIFICITY DEVELOPMENTAL STAGE.