MOUSE ANTI HUMAN CD61

Manufacturer GENWAY
Category
Type Antibody
Specific against Human
Amount 0.1 mg
Host Mouse
ArtNr 20-783-314645
Available
Genway ID:
GWB-9F5811
Specificity:
CD61
Isotype:
IgG1
Preparation:
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution:
Phosphate buffered saline pH7. 4
Preservative Stabilisers:
0. 09% Sodium Azide (NaN3)Approx. Protein Concentrations: IgG concentration 1. 0mg/ml
Immunogen:
PHA stimulated peripheral blood cells.
Specificity:
Recognises CD61 a 105kD glycoprotein which is also known as integrin beta 3 chain. This molecule associates with either the alpha IIb integrin (CD41) or the alpha V integrin (CD51) at the cell surface. CD61 is expressed on platelets and megakaryocytes in association with CD41 and on endothelial cells monocytes platelets and osteoclasts in association with CD51. CD61 is a receptor for fibrinogen fibronectin vWF vitronectin and thrombospondin. Recommended Negative Controls: MOUSE IgG1 NEGATIVE CONTROLRecommended Secondary Antibodies: Rabbit Anti Mouse IgGGoat Anti Mouse IgGGoat Anti Mouse IgG (H/L)Goat Anti Mouse IgG IgA IgMHuCAL Anti Mouse IgG1Goat Anti Mouse IgG (Fc)Sheep Anti Mouse IgG (H/L)
Function:
Integrin alpha-V/beta-3 is a receptor for cytotactin fibronectin laminin matrix metalloproteinase-2 osteopontin osteomodulin prothrombin thrombospondin vitronectin and von Willebrand factor. Integrin alpha-IIb/beta-3 is a receptor for fibronectin fibrinogen plasminogen prothrombin thrombospondin and vitronectin. Integrins alpha-IIb/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha-IIb/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. In case of HIV-1 infection the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi\' s sarcoma lesions. Subunit structureHeterodimer of an alpha and a beta subunit. Beta-3 associates with either alpha-IIb or alpha-V. Isoform Beta-3C interacts with FLNB. Interacts with COMP. Interacts with HIV-1 Tat. Interacts with PDIA6 following platelet stimulation. Ref. 19Ref. 21Ref. 22Ref. 23Subcellular locationMembrane; Single-pass type I membrane protein. Tissue specificityIsoform beta-3A and isoform beta-3C are widely expressed. Isoform beta-3A is specifically expressed in osteoblast cells; isoform beta-3C is specifically expressed in prostate and testis. Post-translational modificationPhosphorylated on tyrosine residues in response to thrombin-induced platelet aggregation. Probably involved in outside-in signaling. A peptide (AA 740-762) is capable of binding GRB2 only when both Tyr-773 and Tyr-785 are phosphorylated. Phosphorylation of Thr-779 inhibits SHC binding. Ref. 18Ref. 20Ref. 26Ref. 29
Polymorphism:
Position 59 is associated with platelet-specific alloantigen HPA-1 (ZW or PL(A)). HPA-1A/ZW(A)/PL(A1) has Leu-59 and HPA-1B/ZW(B)/PL(A2) has Pro-59. HPA-1A is involved in fetal-maternal alloimmune thromobocytopenia (FMAIT) as well as in neonatal alloimmune thrombocytopenia (NAIT). Position 169 is associated with platelet-specific alloantigen HPA-4 (PEN or YUK). HPA-4A/PEN(A)/YUK(A) has Arg-169 and HPA-4B/PEN(B)/YUK(B) has Gln-169. HPA-4B is involved in neonatal alloimmune thrombocytopenia (NAIT or NATP). Position 433 is associated with platelet-specific alloantigen MO. MO- has Pro-433 and MO+ has Ala-433. MO+ is involved in NAIT. Position 515 is associated with platelet-specific alloantigen CA/TU. CA-/TU- has Arg-515 and CA+/TU+ has Gln-515. CA+ is involved in NAIT. Position 662 is associated with platelet-specific alloantigen SR(A). SR(A)- has Arg-662 and SR(A)+ has Cys-662. Involvement in diseaseDefects in ITGB3 are a cause of Glanzmann thrombasthenia (GT) [MIM:273800]; also known as thrombasthenia of Glanzmann and Naegeli. GT is the most common inherited disease of platelets. It is an autosomal recessive disorder characterized by mucocutaneous bleeding of mild-to-moderate severity and the inability of this integrin to recognize macromolecular or synthetic peptide ligands. GT has been classified clinically into types I and II. In type I platelets show absence of the glycoprotein IIb/beta-3 complexes at their surface and lack fibrinogen and clot retraction capability. In type II the platelets express the glycoprotein IIb/beta-3 complex at reduced levels (5-20% controls) have detectable amounts of fibrinogen and have low or moderate clot retraction capability. The platelets of GT \' variants\' have normal or near normal (60-100%) expression of dysfunctional receptors. Ref. 38Ref. 39Ref. 40Ref. 41Ref. 42Ref. 43Ref. 44Ref. 45Ref. 46Ref. 47Ref. 50Ref. 52Ref. 53Ref. 54Ref. 55Ref. 56Ref. 57Sequence similaritiesBelongs to the integrin beta chain family. Contains 1 VWFA domain. [1] \" Molecular cloning and functional expression of a novel CC chemokine receptor cDNA from a human basophilic cell line. \" Power C. A. Meyer A. Nemeth K. Bacon K. B. Hoogewerf A. J. Proudfoot A. E. I. Wells T. N. C. J. Biol. Chem. 270:19495-19500(1995) [PubMed: 7642634] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Spleen. [2] \" New variations of human CC-chemokine receptors CCR3 and CCR4. \" Kato H. Tsuchiya N. Izumi S. Miyamasu M. Nakajima T. Kawasaki H. Hirai K. Tokunaga K. Genes Immun. 1:97-104(1999) [PubMed: 11196669] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] VARIANTS VAL-130 AND SER-178. [3] \" cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www. cdna. org). \" Kopatz S. A. Aronstam R. S. Sharma S. V. Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databasesCited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. [4] \" The status quality and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). \" The MGC Project TeamGenome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Blood. [5] \" The T cell-directed CC chemokine TARC is a highly specific biological ligand for CC chemokine receptor 4. \" Imai T. Baba M. Nishimura M. Kakizaki M. Takagi S. Yoshie O. J. Biol. Chem. 272:15036-15042(1997) [PubMed: 9169480] [Abstract]Cited for: FUNCTION. [6] \" Macrophage-derived chemokine is a functional ligand for the CC chemokine receptor 4. \" Imai T. Chantry D. Raport C. J. Wood C. L. Nishimura M. Godiska R. Yoshie O. Gray P. W. J. Biol. Chem. 273:1764-1768(1998) [PubMed: 9430724] [Abstract]Cited for: FUNCTION. [7] \" The chemokine receptor CCR4 in vascular recognition by cutaneous but not intestinal memory T cells. \" Campbell J. J. Haraldsen G. Pan J. Rottman J. Qin S. Ponath P. Andrew D. P. Warnke R. Ruffing N. Kassam N. Wu L. Butcher E. C. Nature 400:776-780(1999) [PubMed: 10466728] [Abstract]Cited for: FUNCTION. [8] \" Human NK cells express CC chemokine receptors 4 and 8 and respond to thymus and activation-regulated chemokine macrophage-derived chemokine and I-309. \" Inngjerdingen M. Damaj B. Maghazachi A. A. J. Immunol. 164:4048-4054(2000) [PubMed: 10754297] [Abstract]Cited for: FUNCTION TISSUE SPECIFICITY PHOSPHORYLATION.
Amount: 0.1 mg
Available: In stock
Listprice: €326.19
Discount: -10.0%
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