MOUSE ANTI HUMAN SHIP-1 (N-TERMINAL)

GWB-BCD685

IgG2a

Phosphate buffered saline pH7. 4

Synthetic peptide corresponding to a sequence within the N-terminal (NT) region of human SHIP-1.

Phosphatidylinositol (PtdIns) phosphatase that specifically hydrolyzes the 5-phosphate of phosphatidylinositol-3 4 5-trisphosphate (PtdIns(3 4 5)P3) to produce PtdIns(3 4)P2 thereby negatively regulating the PI3K (phosphoinositide 3-kinase) pathways. Acts as a negative regulator of B-cell antigen receptor signaling. Mediates signaling from the FC-gamma-RIIB receptor (FCGR2B) playing a central role in terminating signal transduction from activating immune/hematopoietic cell receptor systems. Acts as a negative regulator of myeloid cell proliferation/survival and chemotaxis mast cell degranulation immune cells homeostasis integrin alpha-IIb/beta-3 signaling in platelets and JNK signaling in B-cells. Regulates proliferation of osteoclast precursors macrophage programming phagocytosis and activation and is required for endotoxin tolerance. Involved in the control of cell-cell junctions CD32a signaling in neutrophils and modulation of EGF-induced phospholipase C activity. Key regulator of neutrophil migration by governing the formation of the leading edge and polarization required for chemotaxis. Modulates FCGR3/CD16-mediated cytotoxicity in NK cells. Mediates the activin/TGF-beta-induced apoptosis through its Smad-dependent expression. May also hydrolyze PtdIns(1 3 4 5)P4 and could thus affect the levels of the higher inositol polyphosphates like InsP6. Ref. 9Ref. 11Catalytic activity1-phosphatidyl-1D-myo-inositol 3 4 5-triphosphate + H2O = 1-phosphatidyl-1D-myo-inositol 3 4-diphosphate + phosphate. Ref. 1Ref. 3Enzyme regulationActivated upon translocation to the sites of synthesis of PtdIns(3 4 5)P3 in the membrane By similarity. Subunit structureInteracts with tyrosine phosphorylated forms of SHC1 DOK1 DOK3 PTPN11/SHP-2 SLAMF1/CD150. Interacts with PTPN11 in response to IL-3. Interacts with receptors EPOR MS4A2/FCER1B and FCER1G FCGR2A FCGR2B and FCGR3. Interacts with GRB2 and PLCG1. Interacts with tyrosine kinases SRC and TEC. Interacts with FCGR2A leading to regulate gene expression during the phagocytic process. Interacts with c-Met/MET By similarity. Ref. 3Ref. 2Ref. 5Ref. 7Ref. 8Subcellular locationCytoplasm. Membrane; Peripheral membrane protein.

The SH2 domain interacts with tyrosine phosphorylated forms of proteins such as SHC1 or PTPN11/SHP-2. It competes with that of GRB2 for binding to phosphorylated SHC1 to inhibit the Ras pathway. It is also required for tyrosine phosphorylation By similarity. The NPXY sequence motif found in many tyrosine-phosphorylated proteins is required for the specific binding of the PID domain By similarity. Post-translational modificationTyrosine phosphorylated by the members of the SRC family after exposure to a diverse array of extracellular stimuli such as cytokines growth factors antibodies chemokines integrin ligands and hypertonic and oxidative stress. Phosphorylated upon IgG receptor FCGR2B-binding. Ref. 5Ref. 8Ref. 10Ref. 12Sequence similaritiesBelongs to the inositol-1 4 5-trisphosphate 5-phosphatase family. Contains 1 SH2 domain. Sequence cautionThe sequence AAC50454. 1 differs from that shown. Reason: Erroneous initiation.