MOUSE ANTI HUMAN CD59:FITC

GWB-A1E1E7

IgG2a

Phosphate buffered saline pH 7. 4

CD59 is an 18-20kD GPI anchored single chain glycoprotein expressed by virtually all cell types including leucocytes platelets and erythrocytes. The antigen binds to C8 and C9 and inhibits assembly of the complement membrane attack complex. Recommended Negative Controls: MOUSE IgG2a NEGATIVE CONTROL:FITC

Soluble form found in a number of tissues. Ref. 12Ref. 16Ref. 18Ref. 19Ref. 20Post-translational modificationN- and O-glycosylated. The N-glycosylation mainly consists of a family of biantennary complex-type structures with and without lactosamine extensions and outer arm fucose residues. Also significant amounts of triantennary complexes (22%). Variable sialylation also present in the Asn-43 oligosaccharide. The predominant O-glycans are mono-sialylated forms of the disaccharide Gal-beta-1 3GalNAc and their sites of attachment are probably on Thr-76 and Thr-77. The GPI-anchor of soluble urinary CD59 has no inositol-associated phospholipid but is composed of seven different GPI-anchor variants of one or more monosaccharide units. Major variants contain sialic acid mannose and glucosamine Sialic acid linked to an N-acetylhexosamine-galactose arm is present in two variants. Ref. 16Ref. 11Ref. 21Glycated. Glycation is found in diabetic subjects but only at minimal levels in nondiabetic subjects. Glycated CD59 lacks MAC-inhibitory function and confers to vascular complications of diabetes. Involvement in diseaseDefects in CD59 are the cause of CD59 deficiency [MIM:612300]. Ref. 25Sequence similaritiesContains 1 UPAR/Ly6 domain.