Antibodies

MOUSE ANTI HUMAN CD4:FITC

Manufacturer GENWAY
Category Products / Antibody
Type Antibody
Specific against Human
Amount 0.02 mg
Host Mouse
ArtNr 20-783-314927
Available
Genway ID:
GWB-9F3172
Specificity:
CD4
Isotype:
IgG1
Preparation:
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution:
Phosphate buffered saline pH7. 4
Preservative Stabilisers:
0. 09% Sodium Azide 1% Bovine Serum AlbuminApprox. Protein Concentrations: IgG concentration 0. 1 mg/ml
Immunogen:
Human PHA blasts
Specificity:
Recognises human CD4 a 55kD cell surface glycoprotein that is primarily expressed on a subpopulation of T lymphocytes on peripheral blood monocytes and on tissue macrophages. Epitope mapping studies have shown that antibodies produced by clone RPA-T4 recognise an epitope within domain 1 of the extracellular region of the CD4 molecule. Clone RPA-T4 has been reported to block gp120-CD4 interaction and inhibit syncytium formation (2). Recommended Negative Controls: MOUSE IgG1 NEGATIVE CONTROL:FITC
Function:
Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts. Subunit structureAssociates with LCK. Binds to HIV-1 gp120 and to P4HB/PDI and upon HIV-1 binding to the cell membrane is part of P4HB/PDI-CD4-CXCR4-gp120 complex. Interacts with HIV-1 Envelope polyprotein gp160 and protein Vpu. Interacts with Human Herpes virus 7 capsid proteins. Ref. 15Ref. 18Ref. 19Ref. 21Subcellular locationCell membrane; Single-pass type I membrane protein.
Note:
Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with and sequesters CD4 in the endoplasmic reticulum. Ref. 20Post-translational modificationPalmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
Miscellaneous:
Primary receptor for HIV-1. Sequence similaritiesContains 3 Ig-like C2-type (immunoglobulin-like) domains. Contains 1 Ig-like V-type (immunoglobulin-like) domain. 1. Moreau J-L. et al. (1987) Monoclonal Antibodies identify three epitope clusters on the mouse p55 subunit of the interleukin 2 receptor: relationship to the interleukin 2 binding site. Eur. J. Immunol. 17:1835 - 18382. Hashimoto N. et al. (1986) Dissociation of interleukin 2 dependent and independent B cell proliferation with monoclonal anti-interleukin 2 receptor antibody. Eur. J. Immunol. 16: 317-320. 3. Lowenthal J. W. et al. (1985) High and low affinity IL-2 receptors: Analysis by IL-2 dissociation rate and reactivity with monoclonal anti-receptor antibody PC61. J. Immunol. 135: 3988-3994. 4. Ceredig R. et al. (1985) Expression of interleukin-2 receptor as a differentiation marker on intrathymic stem cells. Nature 314: 98-100. 5. Yaqoob P. and Calder P. C. (1997) Glutamine Requirement of Proliferating T lymphocytes. Nutrition 13: 646-651. [1] \" Nucleotide sequence and expression of a mouse interleukin 2 receptor cDNA. \" Miller J. Malek T. R. Leonard W. J. Greene W. C. Shevach E. M. Germain R. N. J. Immunol. 134:4212-4217(1985) [PubMed: 3921620] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA]. [2] \" Nucleotide sequence of mouse IL-2 receptor cDNA and its comparison with the human IL-2 receptor sequence. \" Shimuzu A. Kondo S. Takeda S. Yodoi J. Ishida N. Sabe H. Osawa H. Diamantstein T. Nikaido T. Honjo T. Nucleic Acids Res. 13:1505-1516(1985) [PubMed: 2987826] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA]. [3] \" Recombinant lymphokines and their receptors: molecular analysis for the murine interleukin-2 receptor. \" McKereghan K. N. Alpert A. R. Grabstein K. H. Cosman D. Cerretti D. P. Immunol. Ser. 35:109-123(1987)Cited for: NUCLEOTIDE SEQUENCE [MRNA]. [4] \" p55 IL-2 receptor mRNA precursors in murine T lymphocyte nuclei. \" Froussard P. Chastagner P. Somme G. Abadie A. Greene W. Theze J. Longacre S. J. Immunol. 141:1358-1364(1988) [PubMed: 3135330] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-21.
Amount: 0.02 mg
Available: In stock
Listprice: €188.37
Price: €188.37
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