MOUSE ANTI HUMAN CD39:FITC

CD39

Phosphate buffered saline pH7. 4

PHA activated human lymphocytes

In the nervous system could hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. Could also be implicated in the prevention of platelet aggregation. Hydrolyzes ATP and ADP equally well. Ref. 9Catalytic activityATP + 2 H2O = AMP + 2 phosphate. Cofactor: Calcium. Magnesium. Subunit structureHomodimer; disulfide-linked By similarity. Subcellular locationMembrane; Multi-pass membrane protein Potential. Tissue specificityExpressed primarily on activated lymphoid cells. Also expressed in endothelial tissues. The vascular isoform and the placental isoform II are present in both placenta and umbilical vein whereas placental isoform I is present in placenta only. Post-translational modificationThe N-terminus is blocked. Palmitoylated in the N-terminal part. Ref. 11Sequence similaritiesBelongs to the GDA1/CD39 NTPase family. Biophysicochemical properties pH dependence:Optimum pH is 7. 0-7. 5 with ATP as substrate and 7. 5-8. 0 with ADP as substrate. 1. Oppermann M. et al. (1993) Probing the human receptor for C5a anaphylatoxin with site-directed antibodies. Identification of a potential ligand binding site on the NH2-terminal domain. J. Immunol. 151: 3785-94. 2. Oppermann M. et al. (1995) Antibodies from the myeloid panel that react with the C5a receptor and antagonize C5a biological activity. In: Schlossman S. F. (ed. ) Leucocyte Typing V O. U. P: 955-956. 3. Werfel T. et al (1995) Binding of anti-C5a receptor (C5aR) antibodies to cells of clinically normal human skin Leucocyte Typing V. Schlossman S. F. (ed. ) O. U. P. : 957-959. 4. Oppermann M. (1995) Cluster report: (C5a receptor). Leucocyte Typing V. Schlossman S. F. (ed. ) O. U. P. : 953-954. 5. Oppermann M. and Gö tze O. (1994) Plasma clearance of the human C5a anaphylatoxin by binding to leucocyte C5a receptors. Immunology. 82: 516-521. 6. Griebel P. J. et al. (2007) Cross-reactivity of mAbs to human CD antigens with sheep leukocytes. Vet Immunol Immunopathol. 119: 115-22. [1] \" Expression cloning of a receptor for C5a anaphylatoxin on differentiated HL-60 cells. \" Boulay F. Mery L. Tardif M. Brouchon L. Vignais P. Biochemistry 30:2993-2999(1991) [PubMed: 2007135] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA]. [2] \" The chemotactic receptor for human C5a anaphylatoxin. \" Gerard N. P. Gerard C. Nature 349:614-617(1991) [PubMed: 1847994] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. [3] \" cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www. cdna. org). \" Kopatz S. A. Aronstam R. S. Sharma S. V. Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databasesCited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. [4] \" Cloning of human full-length CDSs in BD Creator(TM) system donor vector. \" Kalnine N. Chen X. Rolfs A. Halleck A. Hines L. Eisenstein S. Koundinya M. Raphael J. Moreira D. Kelley T. LaBaer J. Lin Y. Phelan M. Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databasesCited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. [5] \" The DNA sequence and biology of human chromosome 19. \" Grimwood J. Gordon L. A. Olsen A. S. Terry A. Schmutz J. Lamerdin J. E. Hellsten U. Goodstein D. Couronne O. Tran-Gyamfi M. Aerts A. Altherr M. Ashworth L. Bajorek E. Black S. Branscomb E. Caenepeel S. Carrano A. V. Caoile C. Chan Y. M. Christensen M. Cleland C. A. Copeland A. Dalin E. Dehal P. Denys M. Detter J. C. Escobar J. Flowers D. Fotopulos D. Garcia C. Georgescu A. M. Glavina T. Gomez M. Gonzales E. Groza M. Hammon N. Hawkins T. Haydu L. Ho I. Huang W. Israni S. Jett J. Kadner K. Kimball H. Kobayashi A. Larionov V. Leem S. -H. Lopez F. Lou Y. Lowry S. Malfatti S. Martinez D. McCready P. M. Medina C. Morgan J. Nelson K. Nolan M. Ovcharenko I. Pitluck S. Pollard M. Popkie A. P. Predki P. Quan G. Ramirez L. Rash S. Retterer J. Rodriguez A. Rogers S. Salamov A. Salazar A. She X. Smith D. Slezak T. Solovyev V. Thayer N. Tice H. Tsai M. Ustaszewska A. Vo N. Wagner M. Wheeler J. Wu K. Xie G. Yang J. Dubchak I. Furey T. S. DeJong P. Dickson M. Gordon D. Eichler E. E. Pennacchio L. A. Richardson P. Stubbs L. Rokhsar D. S. Myers R. M. Rubin E. M. Lucas S. M. Nature 428:529-535(2004) [PubMed: 15057824] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] VARIANTS ASP-2 AND LYS-279. [6] \" The status quality and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). \" The MGC Project TeamGenome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain. [7] \" Human chemotaxis receptor genes cluster at 19q13. 3-13. 4. Characterization of the human C5a receptor gene. \" Gerard N. P. Bao L. Xiao-Ping H. < href=\" http://www. uniprot. org/uniprot/?query=author:%22Eddy+R. L. +Jr. %22\" rel=\" nofollow\" > Eddy R. L. Jr. Shows T. B. Gerard C. Biochemistry 32:1243-1250(1993) [PubMed: 8383526] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-3. [8] \" Identification of the major phosphorylation sites in human C5a anaphylatoxin receptor in vivo. \" Giannini E. Brouchon L. Boulay F. J. Biol. Chem. 270:19166-19172(1995) [PubMed: 7642584] [Abstract]Cited for: PHOSPHORYLATION AT SER-314; SER-317; SER-327; SER-332; SER-334 AND SER-338. [9] \" Sulfated tyrosines contribute to the formation of the c5a docking site of the human c5a anaphylatoxin receptor. \" Farzan M. Schnitzler C. E. Vasilieva N. Leung D. Kuhn J. Gerard C. Gerard N. P. Choe H. J. Exp. Med. 193:1059-1066(2001) [PubMed: 11342590] [Abstract]Cited for: SULFATION AT TYR-11 AND TYR-14.