MOUSE ANTI HUMAN CD305

GWB-B0C133

IgG1

Phosphate buffered saline pH7. 4

NK cells lines B12. 100 and AM. 25

Functions as an inhibitory receptor that plays a constitutive negative regulatory role on cytolytic function of natural killer (NK) cells B-cells and T-cells. Activation by Tyr phosphorylation results in recruitment and activation of the phosphatases PTPN6 and PTPN11. It also reduces the increase of intracellular calcium evoked by B-cell receptor ligation. May also play its inhibitory role independently of SH2-containing phosphatases. Modulates cytokine production in CD4+ T-cells downregulating IL2 and IFNG production while inducing secretion of transforming growth factor beta. Down-regulates also IgG and IgE production in B-cells as well as IL8 IL10 and TNF secretion. Inhibits proliferation and induces apoptosis in myeloid leukemia cell lines as well as prevents nuclear translocation of NF-kappa-B p65 subunit/RELA and phosphorylation of I-kappa-B alpha/CHUK in these cells. Inhibits the differentiation of peripheral blood precursors towards dendritic cells. Ref. 1Ref. 2Ref. 3Ref. 6Ref. 8Ref. 9Ref. 10Ref. 14Ref. 15Ref. 16Subunit structureInteracts with SH2 domains of tyrosine-protein phosphatases PTPN6 and PTPN11. The interaction with PTPN6 is constitutive. Interacts with the SH2 domain of CSK. Ref. 1Ref. 3Ref. 9Ref. 16Subcellular locationCell membrane; Single-pass type I membrane proteinRef. 1Ref. 2Ref. 9. Tissue specificityExpressed on the majority of peripheral mononuclear cells including natural killer (NK) cells T-cells B-cells monocytes and dendritic cells. Highly expressed in naive T-cells and B-cells but no expression on germinal center B-cells. Abnormally low expression in naive B-cells from HIV-1 infected patients. Very low expression in NK cells from a patient with chronic active Epstein-Barr virus infection. Ref. 15Ref. 7Ref. 12Ref. 13Developmental stageComplete loss of expression when naive B-cells proliferates and differentiates into Ig-producing plasma cells under in vitro stimulation. Ref. 7Induction: By T-cell receptor stimulation in a process that requires p38 MAP kinase and ERK signaling. Ref. 15