MOUSE ANTI HUMAN CD49d

CD49d

Purified IgG prepared by ion exchange chromatography from tissue culture supernatant

Recognises CD49d also known as integrin alpha 4 which associates with either integrin Beta 1 (to form VLA-4) or Beta 7. CD49d is a 150kD molecule which may be cleaved to 80kD and 70kD fragments and is expressed by lymphocytes monocytes eosinophils and thymocytes. VLA-4 mediates cell adhesion to VCAM-1 and fibronectin. Clone 44H6 is reported to induce homotypic aggregation (2). Recommended Negative Controls: MOUSE IgG1 NEGATIVE CONTROLRecommended Secondary Antibodies: Rabbit Anti Mouse IgGGoat Anti Mouse IgGGoat Anti Mouse IgG (H/L)Goat Anti Mouse IgG IgA IgMHuCAL Anti Mouse IgG1Goat Anti Mouse IgG (Fc)Sheep Anti Mouse IgG (H/L) 1. Kilmartin J. V. et al. (1982) Rat monoclonal anti tubulin antibodies derived by using a new non secreting rat cell line. J. Cell Biol. 93: 576-582. 2. Wehland J. et al. (1983) A rat monoclonal antibody reacting specifically with the tyrosylated form of alpha-tubulin. I. Biochemical characterization effects on microtubule polymerization in vitro and microtubule polymerization and organization in vivo J. Cell Biol. 97: 1467-1475. 3. Wallace S. W. et al. (2010) Cdc42 Regulates Apical Junction Formation in Human Bronchial Epithelial Cells through PAK4 and Par6B. Mol Biol Cell. Jul 14. [Epub ahead of print]4. Wehland J. et al. (1984) Amino acid sequence requirements in the epitope recognized by the alpha tubulin specific rat monoclonal antibody YL1/2. EMBO. J. 3: 1295-1300. 5. Burns R. (1987) Cytoskeleton. Tubulin\' s terminal tyrosine Nature 327: 103-104. 6. Skinner R. H. et al. (1991) Use of the Glu-Glu-Phe C-terminal epitope for rapid purification of the catalytic domain of normal and mutant ras GTPase-activating proteins. J. Biol. Chem. 266: 14163-14166.