Mouse Monoclonal Antibody to TNF-alpha

TNF-alpha (tumor necrosis factor alpha) is an important cytokine produced by numerous cell types including neutrophils activated lymphocytes macrophages and NK cells. It plays a critical role in inflammatory responses and in apoptosis. TNF-alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF-alpha exists as a multimer of two three or five non covalently linked units but shows a single 17 kDa band following SDS PAGE under non reducing conditions. Although it has little effect on many cultured normal human cells TNF-alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF-alpha include stimulating growth of human fibroblasts and other cell lines activating polymorphonuclear neutrophils and osteoclasts and induction of interleukin 1 prostaglandin E2 and collagenase production. TNF-alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex.

2A9B9

Western Bloting: 1/500 - 1/2000.
ELISA: Propose dilution 1/10000. Not yet tested in other applications. Determining optimal working dilutions by titration test. 1. Knowlton KU. Yajima T. J Am Coll Cardiol. 2004 Sep 15 44(6):1298-300. 2. Reynolds JL. Ignatowski TA. Gallant S. et al. Brain Res. 2004 Oct 8 1023(1):112-20. 3. Dulak J. Tomala K. Loboda A. et al. Life Sci. 2004 Oct 8 75(21):2573-86.