RAT ANTI MOUSE PLVAP

GWB-A06D42

IgG2a

Phosphate buffered saline pH7. 4

Recognises murine plasmalemma vesicle-associated protein (PLVAP) a 60kD protein which is otherwise known as MECA-32 and PV-1. PLVAP is a transmembrane homodimer which is associated with endothelial cell caveolae and fenestrae. In adult and embryonic mice PLVAP is primarily expressed on endothelial tissues but shows restricted distribution in the skeletal cardiac and brain tissues. In embryonic mice PLVAP expression on the vasculature associated with blood brain barrier has been reported to be developmentally regulated. In humans PLVAP is selectively up-regulated in a variety of high-grade brain tumors including glioblastoma and metastatic carcinoma as well as other cerebral disorders associated with blood-brain barrier disruption such as acute ischemia. PLVAP represents a novel marker of brain tumour angiogenesis and integrity of the blood-brain barrier and is a potential therapeutic target. Recommended Negative Controls: RAT IgG2a NEGATIVE CONTROLRecommended Secondary Antibodies: Goat Anti Rat IgGRabbit Anti Rat IgGGoat Anti Rat IgG (MOUSE ADSORBED)

Membrane-associated protein of caveolae. Found in fenestral and stomatal diaphragms in fenestrated endothelia and transendothelial channels. Also colocalized with CAV1 in perinuclear region By similarity. Tissue specificityExpressed in lung kidney spleen heart muscle eye pancreas thyroid thymus submaxillary gland prostate epididymis uterus and liver. Ref. 1Ref. 2Developmental stageExpressed in embryo at 7 dpc. Expressed in the brain vasculature at 12 up to 16 dpc whereupon it disappears. Ref. 1Ref. 2 [1] \" Human interleukin-10-related T cell-derived inducible factor: molecular cloning and functional characterization as an hepatocyte-stimulating factor. \" Dumoutier L. Van Roost E. Colau D. Renauld J. -C. Proc. Natl. Acad. Sci. U. S. A. 97:10144-10149(2000) [PubMed: 10954742] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA]. [2] \" IL-TIF/IL-22: genomic organization and mapping of the human and mouse genes. \" Dumoutier L. Van Roost E. Colau D. Ameye G. Michaux L. Renauld J. -C. Genes Immun. 1:488-494(2000) [PubMed: 11197690] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. [3] \" Interleukin (IL)-22 a novel human cytokine that signals through the interferon receptor-related proteins CRF2-4 and IL-22R. \" Xie M. -H. Aggarwal S. Ho W. -H. Foster J. Zhang Z. Stinson J. Wood W. I. Goddard A. D. Gurney A. L. J. Biol. Chem. 275:31335-31339(2000) [PubMed: 10875937] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA]. [4] \" The secreted protein discovery initiative (SPDI) a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment. \" Clark H. F. Gurney A. L. Abaya E. Baker K. Baldwin D. T. Brush J. Chen J. Chow B. Chui C. Crowley C. Currell B. Deuel B. Dowd P. Eaton D. Foster J. S. Grimaldi C. Gu Q. Hass P. E. Heldens S. Huang A. Kim H. S. Klimowski L. Jin Y. Johnson S. Lee J. Lewis L. Liao D. Mark M. R. Robbie E. Sanchez C. Schoenfeld J. Seshagiri S. Simmons L. Singh J. Smith V. Stinson J. Vagts A. Vandlen R. L. Watanabe C. Wieand D. Woods K. Xie M. -H. Yansura D. G. Yi S. Yu G. Yuan J. Zhang M. Zhang Z. Goddard A. D. Wood W. I. Godowski P. J. Gray A. M. Genome Res. 13:2265-2270(2003) [PubMed: 12975309] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. [5] SeattleSNPs variation discovery resourceSubmitted (JUN-2001) to the EMBL/GenBank/DDBJ databasesCited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] VARIANT GLY-158. [6] \" The status quality and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). \" The MGC Project TeamGenome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Blood. [7] \" Signal peptide prediction based on analysis of experimentally verified cleavage sites. \" Zhang Z. Henzel W. J. Protein Sci. 13:2819-2824(2004) [PubMed: 15340161] [Abstract]Cited for: PROTEIN SEQUENCE OF 34-48. [8] \" Crystallization and synchrotron X-ray diffraction studies of human interleukin-22. \" Nagem R. A. Lucchesi K. W. Colau D. Dumoutier L. Renauld J. -C. Polikarpov I. Acta Crystallogr. D 58:529-530(2002) [PubMed: 11856845] [Abstract]Cited for: X-RAY CRYSTALLOGRAPHY (2. 0 ANGSTROMS) OF 29-179. [9] \" Structure of insect-cell-derived IL-22. \" Xu T. Logsdon N. J. Walter M. R. Acta Crystallogr. D 61:942-950(2005) [PubMed: 15983417] [Abstract]Cited for: X-RAY CRYSTALLOGRAPHY (2. 6 ANGSTROMS) OF 38-179 GLYCOSYLATION AT ASN-54 AND ASN-97. [10] \" Structure of IL-22 bound to its high-affinity IL-22R1 chain. \" Jones B. C. Logsdon N. J. Walter M. R. Structure 16:1333-1344(2008) [PubMed: 18599299] [Abstract]Cited for: X-RAY CRYSTALLOGRAPHY (2. 5 ANGSTROMS) OF 39-179 IN COMPLEX WITH IL22RA1 DISULFIDE BONDS.