RAT ANTI MOUSE CD98

Manufacturer GENWAY
Category
Type Antibody
Specific against Mouse
Amount 0.1 mg
Host Rat
ArtNr 20-783-314679
Available
Genway ID:
GWB-A09006
Specificity:
CD98
Isotype:
IgG1
Preparation:
Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution:
Phosphate buffered saline pH7. 4
Preservative Stabilisers:
0. 09% Sodium Azide (NaN3)Approx. Protein Concentrations: IgG concentration 1. 0mg/ml
Immunogen:
Thymic epithelial cells from mouse
Specificity:
Recognises murine CD98 (also known as 4F2 and SLC3A2) a type II transmembrane glycoprotein which associates with members of the L-type amino transporter family to form a heterodimeric complex. CD98 constitutes the heavy chain of the complex and is required for the surface expression and basolateral localization of the amino acid transporter complex in polarized epithelial cells. The CD98 heavy chain also interacts with beta 1 integrins and regulates their activation through the cytoplasmic domains and transmembrane regions. Studies suggest that overexpression of CD98 may contribute to cell growth and survival by regulating integrin signalling and therefore may play an important role in tumorigenesis. Recommended Negative Controls: RAT IgG1 NEGATIVE CONTROLRecommended Secondary Antibodies: Goat Anti Rat IgGRabbit Anti Rat IgGGoat Anti Rat IgG (MOUSE ADSORBED)
Function:
Required for the function of light chain amino-acid transporters. Involved in sodium-independent high-affinity transport of large neutral amino acids such as phenylalanine tyrosine leucine arginine and tryptophan. Involved in guiding and targeting of LAT1 and LAT2 to the plasma membrane. When associated with SLC7A6 or SLC7A7 acts as an arginine/glutamine exchanger following an antiport mechanism for amino acid transport influencing arginine release in exchange for extracellular amino acids. Plays a role in nitric oxide synthesis in human umbilical vein endothelial cells (HUVECs) via transport of L-arginine. Required for normal and neoplastic cell growth. When associated with SLC7A5/LAT1 is also involved in the transport of L-DOPA across the blood-brain barrier and that of thyroid hormones triiodothyronine (T3) and thyroxine (T4) across the cell membrane in tissues such as placenta. Involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine or D L-homocysteine complexes and hence plays a role in metal ion homeostasis and toxicity. When associated with SLC7A5 or SLC7A8 involved in the cellular activity of small molecular weight nitrosothiols via the stereoselective transport of L-nitrosocysteine (L-CNSO) across the transmembrane. Together with ICAM1 regulates the transport activity LAT2 in polarized intestinal cells by generating and delivering intracellular signals. When associated with SLC7A5 plays an important role in transporting L-leucine from the circulating blood to the retina across the inner blood-retinal barrier. Ref. 6Ref. 7Ref. 9Subunit structureDisulfide-linked heterodimer of a glycosylated heavy chain and a non-glycosylated light chain (SLC7A5 SLC7A6 SLCA7A7 SLC7A8 SLC7A10 or SLCA7A11). Colocalizes with cadherins By similarity. Interacts with FAM57A/CT120 and ICAM1 By similarity. Constitutively and specifically associates with beta-1 integrins (alpha-2/beta-1 alpha-3/beta-1 alpha-5/beta-1 and alpha-6/beta-1) but minimally with alpha-4/beta-1 By similarity. Ref. 6Ref. 7Ref. 8Subcellular locationApical cell membrane; Single-pass type II membrane protein. Melanosome By similarity.
Note:
Identified by mass spectrometry in melanosome fractions from stage I to stage IV By similarity. Localized to the plasma membrane when associated with SLC7A5 or SLC7A8 By similarity. Localized to the placental apical membrane By similarity. Located selectively at cell-cell adhesion sites. Colocalized with SLC7A8/LAT2 at the basolateral membrane of kidney proximal tubules and small intestine epithelia. Expressed in both luminal and abluminal membranes of brain capillary endothelial cells By similarity. Ref. 6Ref. 8Tissue specificityObserved in all adult tissues tested with strongest expression in kidney small intestine spleen thymus and liver. Moderate expression in brain stomach heart testis lung skin pancreas and skeletal muscle. Expressed on the surface of epithelial cells of most embryonic tissues including epidermis choroid plexus in the brain retina as well as intestinal renal and thymic epithelium. In brain expressed on capillary endothelia in cerebral cortex. Ref. 6Ref. 8Ref. 1Developmental stageStrong expression in the liver of 14 dpc embryo and in brain spleen liver kidney and intestine of an 18 dpc embryo. Ref. 6Induction: Expression induced by concanavilin-A stimulation. Induced during cell activation but is subsequently maintained at constant levels throughout the cell cycle in exponentially growing cells. Ref. 6Ref. 1Post-translational modificationPhosphorylation on Ser-300 or Ser-302 and on Ser-420 by ecto-protein kinases favors heterotypic cell-cell interactions By similarity.
Miscellaneous:
Leucine uptake was inhibited by ileum valine histidine and phenylalanine as well as by 2-aminobicyclo-(2 2 1)-heptane-2-carboxylic acid (BCH) (a specific inhibitor of system L transport). Sequence similaritiesBelongs to the SLC3A transporter family. [1] \" A new protein conjugation system in human. The counterpart of the yeast Apg12p conjugation system essential for autophagy. \" Mizushima N. Sugita H. Yoshimori T. Ohsumi Y. J. Biol. Chem. 273:33889-33892(1998) [PubMed: 9852036] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1) CONJUGATION TO ATG5 TISSUE SPECIFICITY. [2] \" The status quality and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). \" The MGC Project TeamGenome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Brain and Ovary.
Amount: 0.1 mg
Available: In stock
Listprice: €242.60
Discount: -10.0%
Price: €218.34
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