PARP2 (poly (ADP-ribose) polymerase family. member 2)

Polyclonal antibody produced in rabbits immunized with a synthetic peptide corresponding to a region of Human PARP2.

Suggested starting concentrations are provided. Optimal dilutions should be determined by end-user. Differences in calculated versus apparent molecular weight may be due to post-translational modifications or protein hydrophobicity. PARP2 contains a catalytic domain and is capable of catalyzing a poly(ADP-ribosyl)ation reaction. This protein has a catalytic domain which is homologous to that of poly (ADP-ribosyl) transferase. but lacks an N-terminal DNA binding domain which activates

NAD+ + (ADP-D-ribosyl)(n)-acceptor = nicotinamide + (ADP-D-ribosyl)(n+1)-acceptor.

Nucleus.

Poly-ADP-ribosylated by PARP1 (By similarity).

Contains 1 PARP catalytic domain. Summary: This protein is poly(ADP-ribosyl)transferase-like 2 protein which contains a catalytic domain and is capable of catalyzing a poly(ADP-ribosyl)ation reaction. This protein has a catalytic domain which is homologous to that of poly (ADP-ribosyl) transferase but lacks an N-terminal DNA binding domain which activates the C-terminal catalytic domain of poly (ADP-ribosyl) transferase. The basic residues within the N-terminal region of this protein may bear potential DNA-binding properties and may be involved in the nuclear and/or nucleolar targeting of the protein. Two alternatively spliced transcript variants encoding distinct isoforms have been found. Malanga. M. et al. . (2004) J. Biol. Chem. 279 (7). 5244-5248.