PIAS2 (protein inhibitor of activated STAT 2)

Polyclonal antibody produced in rabbits immunized with a synthetic peptide corresponding to a region of Mouse Pias2.

TF for Mouse

Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase stabilizing the interaction between UBE2I and the substrate and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways including the STAT pathway the p53 pathway and the steroid hormone signaling pathway. The effects of this transcriptional coregulation transactivation or silencing may vary depending upon the biological context and PIAS2 isoform studied. However it seems to be mostly involved in gene silencing. Binds to sumoylated ELK1 and enhances its transcriptional activity by preventing recruitment of HDAC2 by ELK1 thus reversing SUMO-mediated repression of ELK1 transactivation activity. Isoform PIASx-beta but not isoform PIASx-alpha promotes MDM2 sumoylation. Isoform PIASx-alpha promotes PARK7 sumoylation. Isoform PIASx-beta promotes NCOA2 sumoylation more efficiently than isoform PIASx-alpha (By similarity).

Binds SUMO1 and UBE2I. Interacts with JUN MDM2 PARK7 TP53 and TP73 isoform alpha but not TP73 isoform beta. Interacts with STAT4 following IL12 and IFN-alpha stimulation of T-cells. Interacts also with GTF2I GTF2IRD1 DAB2 and MSX2 as well as with several steroid receptors including ESR1 ESR2 NR3C1 PGR AR and with NCOA2. Sumoylation of a target protein seems to enhance the interaction. Binds to sumoylated ELK1. Interacts with PLAG1 (By similarity). Binds DNA such as CDKN1A promoter in a sequence-specific manner.

The LXXLL motif is a transcriptional coregulator signature.

Belongs to the PIAS family.

Contains 1 SP-RING-type zinc finger. [1] Wu L. Wu H. Ma L. Sangiorgi F. Wu N. Bell J. R. Lyons G. E. and Maxson R. Miz1 a novel zinc finger transcription factor that interacts with Msx2 and enhances its affinity for DNA[2] Wu L. Wu H. Sangiorgi F. Wu N. Bell J. R. Lyons G. E. and Maxson R. Direct Submission[3] Cho S. -Y. Jeon J. W. Lee S. H. Park S. -S. p67 isoform of mouse disabled 2 protein acts as a transcriptional activator during the differentiation of F9 cells. [4] Carninci P. Kasukawa T. Katayama S. Gough J. Frith M. C. Maeda N. Oyama R. Ravasi T. Lenhard B. Wells C. et al. The transcriptional landscape of the mammalian genome. [5] Wu L. Wu H. Ma L. Sangiorgi F. Wu N. Bell J. R. Lyons G. E. Maxson R. Miz1 a novel zinc finger transcription factor that interacts with Msx2 and enhances its affinity for DNA. [6] Wu L. Wu H. Ma L. Sangiorgi F. Wu N. Bell J. R. Lyons G. E. Maxson R. Mech. Dev. 69:219-219(1997). [7] Tussie-Luna M. I. Michel B. Hakre S. Roy A. L. The SUMO ubiquitin-protein isopeptide ligase family member Miz1/PIASxbeta/Siz2 is a transcriptional cofactor for TFII-I. [8] Kotaja N. Karvonen U. Jaenne O. A. Palvimo J. J. PIAS proteins modulate transcription factors by functioning as SUMO-1 ligases.