MNAT1 (menage a trois homolog 1. cyclin H assembly factor (Xenopus laevis))

Polyclonal antibody produced in rabbits immunized with a synthetic peptide corresponding to a region of Human MNAT1. Western Blot: Suggested dilution at 1. 25 microg/ml in 5% skim milk / PBS buffer. and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50. 000 - 100. 000 as second antibody.

Suggested starting concentrations are provided. Optimal dilutions should be determined by end-user. Differences in calculated versus apparent molecular weight may be due to post-translational modifications or protein hydrophobicity. Cyclin-dependent kinases (CDKs) which play an essential role in cell cycle control of eukaryotic cells are phosphorylated and thus activated by the CDK-activating kinase (CAK). CAK is a multisubunit protein that includes CDK7 cyclin H and MAT1. MAT1 (for \' menage a trois-1\' ) is involved in the assembly of the CAK complex. Cyclin-dependent kinases (CDKs) which play an essential role in cell cycle control of eukaryotic cells are phosphorylated and thus activated by the CDK-activating kinase (CAK). CAK is a multisubunit protein that includes CDK7 (MIM 601955) cyclin H (CCNH; MIM 601953) and MAT1. MAT1 (for \' menage a trois-1\' ) is involved in the assembly of the CAK complex. [supplied by OMIM].

Associates primarily with CDK7 and cyclin H to form the CAK complex. CAK can further associate with the core-TFIIH to form the TFIIH basal transcription factor.

Highest levels in colon and testis. Moderate levels are present thymus prostate ovary and small intestine. The lowest levels are found in spleen and leukocytes.

Contains 1 UIM (ubiquitin-interacting motif) repeat. Summary: Cyclin-dependent kinases (CDKs) which play an essential role in cell cycle control of eukaryotic cells are phosphorylated and thus activated by the CDK-activating kinase (CAK). CAK is a multisubunit protein that includes CDK7 (MIM 601955) cyclin H (CCNH; MIM 601953) and MAT1. MAT1 (for \' menage a trois-1\' ) is involved in the assembly of the CAK complex. [supplied by OMIM]. Zhou. M. . (2003) Proc. Natl. Acad. Sci. U. S. A. 100 (22). 12666-12671.