RFC5

IgG

Human

RFC5

Tris-citrate/phosphate pH 7-8 0. 1% sodium azide

36 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC also named activator 1 is a protein complex consisting of five distinct subunits of 140 40 38 37 and 36 kD. This gene encodes the 36 kD subunit. This subunit can interact with the C-terminal region of PCNA. It forms a core complex with the 38 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

Heterotetramer of subunits RFC2 RFC3 RFC4 and RFC5 that can form a complex either with RFC1 or with RAD17. The former interacts with PCNA in the presence of ATP while the latter has ATPase activity but is not stimulated by PCNA.

Belongs to the activator 1 small subunits family.