Actin smooth muscle

IgG

Human

Actin smooth muscle

0. 2 mg/ml Storage

ELISA: Use at a concentration of 0. 1 - 1. 0 µ g/ml. IHC: Use at a dilution of 1/100 - 1/400. WB: Use at a concentration of 0. 5 - 2. 0 µ g/ml. Predicted

Cytoplasmic Actins are highly conserved proteins expressed in all eucaryotic cells. Actin filaments form part of the cytoskeleton and play essential roles in regulating cell shape and movement. Six distinct actin isotypes have been identified in mammalian cells. Each is encoded by a separated gene and is expressed in a developmentally regulated and tissue-specific manner alpha and beta cytoplasmic actins are expressed in a wide variety of cells; whereas expression of alpha skeletal alpha cardiac alpha vascular and gamma enteric actins are more restricted to specialized muscle cell type. Smooth muscle alpha actin is of further interest because it is one of a few genes whose expression is relatively restricted to vascular smooth muscle cells. Further more expression of smooth muscle alpha actin is regulated by hormones cell proliferation and altered by pathological conditions including oncogenic transformation and atherosclerosis.

Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others.

In vertebrates 3 main groups of actin isoforms alpha beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.