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PKC beta 2 phospho T641

PKC beta 2 phospho T641

Item no.	18-272-197472
Manufacturer	GENWAY
Amount	0.05 ml
Category	Metabolism Diabetes Antibodies Western Blot Primary Antibodies
Type	Antibody
Applications	WB
Specific against	other
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	GWB-DB6257
Similar products	18-272-197472
Available
Genway ID:	GWB-DB6257
Isotype:	IgG
Immunogen:	Synthetic phosphopeptide derived from a region of human PKC beta 2 that contains threonine 641.
Antigen Species:	Human
Positive Control:	K562 cells treated with PMA a phorbol ester
Target:	PKC beta 2
Localization:	Cytoplasmic and membrane associated.
Purification Note:	The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated PKC beta 2. The final product is generated by affinity chromatography using a PKC beta 2-derived peptide that is phosphorylated at threonine 641. Storage
Preservative:	0. 05% Sodium Azide; Constituents: 50% Glycerol PBS 1mg/ml BSA. pH 7. 3
Application Note:	WB: Use at a dilution of 1/1000. Detects a band of approximately 80 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. Cellular
Localization:	Cytoplasmic and membrane associated. Protein Kinase C beta 2 (PKC beta 2) is an 80 kDa member of the conventional group (cPKCs: sensitive to diacylglycerol phosphotidylserine and phorbol esters) of the PKC family of serine/threonine kinases that are involved in a wide range of physiological processes including mitogenesis cell survival transcriptional regulation and tumor promotion. PKC beta 2 is phosphorylated on three sites threonine 500 in the activation loop threonine 641 in the turn loop and serine 660 in the hydrophobic loop. Threonine 641 mediates PKC beta 2 binding to Hsp70 which regulates its stability and phosphorylation. Phosphorylation of threonine 641 is also crucial for PKC beta 2 proper subcellular localization and catalytic function as well as for its autophosphorylation on serine 660.
Function:	This is a calcium-activated phospholipid-dependent serine- and threonine-specific enzyme. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters a class of tumor promoters. May be considered as a novel component of the NF-kappa-B signaling axis responsible for the survival and activation of B-cells after BCR cross-linking (By similarity).
Catalytic Activity:	ATP + a protein = ADP + a phosphoprotein. Cofactor: Binds 3 calcium ions per subunit. The ions are bound to the C2 domain (By similarity).
Subunit:	Interacts with PDK1 (By similarity). Interacts in vitro with PRKCBP1.
Subcellular Location:	Cytoplasm (By similarity). Membrane; Peripheral membrane protein (By similarity).
Ptm:	Phosphorylation on Thr-500 of isoform beta-I within the activation loop renders it competent to autophosphorylate. Subsequent autophosphorylation of Thr-642 maintains catalytic competence and autophosphorylation on Ser-661 appears to release the kinase into the cytosol. Similarly isoform beta-II is autophosphorylated on \' Thr-640\' and \' Ser-659\' subsequent to phosphorylation on Thr-500. Autophosphorylated on other sites i. e. in the N-terminal and hinge regions have no effect on PKC activity (By similarity).
Similarity:	Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Similarity:	Contains 1 AGC-kinase C-terminal domain.
Similarity:	Contains 1 C2 domain.
Similarity:	Contains 2 phorbol-ester/DAG-type zinc fingers.
Similarity:	Contains 1 protein kinase domain.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 0.05 ml

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Delivery expected until 10/30/2025

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