RFX5

GWB-94EC6F

Immunogen was a synthetic peptide which represented a portion of human Regulatory Factor X 5 encoded within exon 5 (LocusLink ID 5993) conjugated to KLH. After multiple immunizations in Freund\' s adjuvant serum was collected. Antibodies were affinity purified using the peptide immobilized on solid support.

Reacts with RFX5

1 mg/ml

absorbance at 280 nmof 1. 4 equals 1. 0 mg of IgG. Storage

For IP: Use at a concentration of 1 - 4 µ l. WB: Use at a dilution of 1:500 - 1:5000. Not tested in other applications. Optimal dilutions/concentrations should be determined by the researcher. RFX5 is the regulatory gene responsible for human MHC class II deficiency. Patients with this disease exhibit a characteristic defect in the binding of a nuclear factor RFX to the X box motif of MHC class II promoters. RFX5 is the 75 kDa subunit of the RFX complex which is absolutely essential and highly specific for regulating MHC class II gene expression and control of the immune response. RFX5 is a protein consisting of 616 amino acids.

RFX consists of at least three different subunits; RFXAP RFX5 and RFX-B/RFXANK; with each subunit representing a separate complementation group. RFX forms cooperative DNA binding complexes with X2BP and CBF/NF-Y. RFX associates with CIITA to form an active transcriptional complex.

Ubiquitous.

Phosphorylated.

Belongs to the RFX family.