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Hepatitis B surface antigen (HRP)

Hepatitis B surface antigen (HRP)

Item no.	18-272-197927
Manufacturer	GENWAY
Amount	0.1 ml
Category	Oncology Antibodies ELISA Primary Antibodies By Organ Hepatic Cancer
Type	Antibody
Applications	ELISA
Specific against	other
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	GWB-475DB7
Similar products	18-272-197927
Available
Genway ID:	GWB-475DB7
Isotype:	IgG
Immunogen:	Hepatitis B surface antigen purified from human serum. Mixture of subtypes ad & ay.
Antigen Species:	Human
Specificity:	Monospecific reacts only with Hepatitis B surface antigen including the pre-S1 epitope. Non-reactive with normal human serum.
Target:	Hepatitis B surface antigen
Localization:	Secreted
Conjugation:	HRP
Concentration:	1 mg/ml
Purification Note:	Protein A chromatography purified IgG fraction covalently coupled to a highly purified preparation of horseradish peroxidase (RZ> 3). Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Free enzyme is absent. Storage
Preservative:	0. 002% Thimerosal (merthiolate). Constituents: PBS 10mg/ml BSA
Application Note:	ELISA: Use at an assay dependent dilution. Suitability for use in Western blot IHC and IFA has not been determined but use in such assays should not be excluded. allNot tested in other applications. Optimal dilutions/concentrations should be determined by the end user. Cellular
Localization:	Secreted Hepatitis B Virus (HBV) infection induces a disease state characterised by liver damage inflammation and viral persistence. Infection also increases the risk of hepatocellular carcinoma. HBV belongs to the Hepadnaviridae family of viruses. Its genome consists of partially double stranded circular DNA. The DNA is enclosed in a nucleocapsid or core antigen (HBcAg) which is surrounded by a spherical envelope (surface antigen or HBsAg). The core antigen shares its sequences with the e antigen (HBeAg) but no cross reactivity between the two proteins has been observed. The HBV genome also encodes a DNA polymerase that also acts as a reverse transcriptase. Hepatitis B infection is normally diagnosed from serological tests that detect HBsAg but as the disease progresses this antigen may no longer be present in the blood and tests for HBcAg are used. If HBsAg can be detected in the blood for longer than six months chronic hepatitis B is diagnosed. The antigenic determinant of the protein moiety of the HBsAg determines specific characteristics of different serotypes and provides the basis of immunodetection. HBsAg has antigenic heterogeneity specifically two pairs of sub specific determinants d/y and w/r allow the following combinations: adw ayw adr ayr.
Function:	The large envelope protein exists in two topological conformations one which is termed \' external\' or Le-HBsAg and the other \' internal\' or Li-HBsAg. In its external conformation the protein attaches the virus to cell receptors and thereby initiating infection. This interaction determines the species specificity and liver tropism. It also assume fusion between virion membrane and cellular membrane. In its internal conformation the protein plays a role in virion morphogenesis and mediates the contact with the nucleocapsid like a matrix protein (By similarity).
Function:	The middle envelope protein plays an important role in the budding of the virion. It is involved in the induction of budding in a nucleocapsid independent way. In this process the majority of envelope proteins bud to form subviral lipoprotein particles of 22 nm of diameter that do not contain a nucleocapsid (By similarity).
Subunit:	Li-HBsAg interacts with capsid protein and with HDV Large delta antigen. Isoform M associates with host chaperone CANX through its pre-S2 N glycan. This association may be essential for M proper secretion (By similarity).
Subcellular Location:	Virion membrane (By similarity).
Domain:	The large envelope protein is synthesized with the pre-S region at the cytosolic side of the endoplasmic reticulum and hence will be within the virion after budding. Therefore the pre-S region is not N-glycosylated. Later a post-translational translocation of N-terminal pre-S and TM1 domains occur in about 50% of proteins at the virion surface. These molecules change their topology by an unknown mechanism resulting in exposure of pre-S region at virion surface. For isoform M in contrast the pre-S2 region is translocated cotranslationally to the endoplasmic reticulum lumen and is N-glycosylated.
Ptm:	Isoform M is N-terminaly acetylated at a ratio of 90% N- and O-glycosylated at the pre-S2 region.
Ptm:	Myristoylated (By similarity). Biotechnology: Systematic vaccination of individuals at risk of exposure to the virus has been the main method of controlling the morbidity and mortality associated with hepatitis B. The first hepatitis B vaccine was manufactured by the purification and inactivation of HBsAg obtained from the plasma of chronic hepatitis B virus carriers. The vaccine is now produced by recombinant DNA techniques and expression of the S isoform in yeast cells. The pre-S region do not seem to induce strong enough antigenic response.
Similarity:	Belongs to the orthohepadnavirus major surface antigen family.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 0.1 ml

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Delivery expected until 9/11/2025

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