Influenza A M1 protein*B1G1

IgG

Purified matrix protein (M) recognizes the matrix protein for any strain of Influenza A conservation of the matrix protein sequence between hemagluttinin/Neuraminidase typed strains does not react with HEp-2 cells by indirect immunofluorescence

Cytoplasmic

From polyclonal serum. Storage

Use at an assay dependent dilution.
ELISA: Use at an assay dependent dilution. IF: Use at an assay dependent dilution. Is unsuitable for IHC. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. Cellular

Plays critical roles in virus replication from virus entry and uncoating to assembly and budding of the virus particle. M1 binding to ribonucleocapsids (RNPs) in nucleus seems to inhibit viral transcription. Interaction of viral NEP with M1-RNP is thought to promote nuclear export of the complex which is targeted to the virion assembly site at the apical plasma membrane in polarized epithelial cells. Interactions with NA and HA may bring M1 a non-raft-associated protein into lipid rafts. Forms a continuous shell on the inner side of the lipid bilayer in virion where it binds the RNP. During virus entry into cell the M2 ion channel acidifies the internal virion core inducing M1 dissociation from the RNP. M1-free RNPs are transported to the nucleus where viral transcription and replication can take place.

Homodimer and homomultimer. Interacts with NEP. Binds ribonucleocapsid by both interacting with genomic RNA and NP protein. May interact with HA and NA (By similarity). Cannot bind NP without genomic RNA.

Most abundant protein in virion. When expressed alone can form virus-like particles in transfected cells.