Ku70

Item no.	18-272-198050
Manufacturer	GENWAY
Amount	0.1 ml
Category	Oncology Antibodies By Cell Type Carcinoma Melanoma Western Blot Primary Antibodies
Type	Antibody
Applications	WB
Specific against	other
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	GWB-BEEAD1
Similar products	18-272-198050
Available
Genway ID:	GWB-BEEAD1
Isotype:	IgG
Immunogen:	Recombinant antigen containing the amino terminal portion of human Ku70 protein
Antigen Species:	Human
Positive Control:	HeLa Raji
Target:	Ku70
Localization:	Nuclear
Concentration:	Antiserum Storage
Buffer:	Phosphate-buffered saline pH 7. 4 containing no preservatives
Application Note:	For WB: Use at a dilution of 1:2 500. Not tested in other applications. Optimal dilutions/concentrations should be determined by the researcher. Target protein
Molecular Weight:	70 Cellular
Localization:	Nuclear Originally identified as an autoantigen the Ku protein are nuclear proteins found in eukaryotes. They exist as a heterodimer consisiting of Ku 70 and Ku 80 migrating at approximately 70 and 80 kDa respectively. They bind to DNA ends at double strand breaks. The Ku proteins interact with the DNA-dependent protein kinase (DNA-PK) and participate in the targeting of DNA-PK to DNA. The Ku proteins may participate in numerous cellular processes including DNA repair and replication cell signalling cell proliferation transcriptional activation and apoptosis.
Function:	Single stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3\' -5\' direction. Binding to DNA may be mediated by p70. Involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The Ku p70/p86 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The Ku p70/p86 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. Required for osteocalcin gene expression (By similarity).
Subunit:	Heterodimer of a 70 kDa and a 80 kDa subunit. The dimer associates in a DNA-dependent manner with PRKDC to form the DNA-dependent protein kinase complex DNA-PK and with the LIG4-XRCC4 complex. The dimer also associates with NARG1 and this complex binds to the osteocalcin promoter and activates osteocalcin expression. In addition the 70 kDa subunit binds to the osteoblast-specific transcription factors MSX2 RUNX2 and DLX5. Interacts with ELF3. Interactis with XRCC6BP1.
Subcellular Location:	Nucleus. Developmental Stage: Expression does not increase during promyelocyte differentiation.
Induction:	In osteoblasts by FGF2.
Ptm:	Phosphorylation by PRKDC may enhance helicase activity. Phosphorylation of Ser-51 does not affect DNA repair.
Disease:	Individuals with systemic lupus erythematosus (SLE) and related disorders produce extremely large amounts of autoantibodies to p70 and p86. Existence of a major autoantigenic epitope or epitopes on the C-terminal 190 amino acids of p70 containing the leucine repeat. The majority of autoantibodies to p70 in most sera from patients with SLE seem to be reactive with this region.
Similarity:	Contains 1 Ku domain.
Similarity:	Contains 1 SAP domain.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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