Anti-Apoptosis Inducing Factor (AIF)

Apoptosis Inducing Factor (AIF)

This antibody has been tested for use in western blotting and immunohistochemistry. K562 cell lysate as well as rat and mouse heart tissue lysates can be used as positive controls in western blotting and a band at approximately 67 kDa is expected. Specific conditions for reactivity should be optimized by the end user. Recommended

0. 02 M Potassium Phosphate 0. 15 M Sodium Chloride pH 7. 2

Dry Ice Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of the caspase family caspase activated DNase and several novel proteins (1). A novel gene the product of which causes chromatin condensation and DNA fragmentation was recently identified cloned and designated apoptosis inducing factor (AIF) (2). Like cytochrome c and caspase-9 which are critical molecules in apoptosis AIF localizes to mitochondria. AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. AIF induces chromatin condensation and large scale DNA fragmentation which are the hallmarks of apoptosis. These effects occur in both isolated nuclei and in the nuclei of live cells treated by microinjection and with apoptosis stimuli (2 3). AIF is highly conserved between human and mouse and is widely expressed (2). 1. Zamzami N Kroemer G (1999) Condensed matter in cell death. Nature 401:127-128. 2. Susin SA Lorenzo HK Zamzami N et al. (1999) Molecular characterization of mitochondrial apoptosisinducing factor. Nature 397:441-446. 3. Daugas E Susin SA Zamzami N et al. (2000) Mitochondrio-nuclear translocation of AIF in apoptosis and necrosis. FASEB J. 14:729-739.