Anti-Toll-like Receptor 1 (TLR1)

TLR1

This IgG fraction antibody has been tested for use in ELISA immunohistochemistry and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~90 kDa in size corresponding to TLR1 by western blotting in the appropriate cell lysate or extract. Recommended

This IgG fraction antibody is directed against human TLR1 protein. This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This antibody is reactive with TLR1 from human and mouse sources. Cross reactivity with TLR1 from other sources has not been determined. Antibody

0. 02 M Potassium Phosphate 0. 15 M Sodium Chloride pH 7. 2

Dry Ice Toll-like receptors (TLRs) are evolutionarily conserved pattern-recognition molecules resembling the toll proteins that mediate antimicrobial responses in Drosophila. These proteins recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. The TLRs act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors so the organism can respond to potential infection. TLR1 is co-expressed with TLR2 on myeloid cells of the innate immune systems in lymphoid tissue such as monocytes and dendritic cells where they form heterodimers that can recognize triacylated lipoproteins. Summary: The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This protein is ubiquitously expressed and at higher levels than other TLR genes. Different length transcripts presumably resulting from use of alternative polyadenylation site and/or from alternative splicing have been noted for this gene. [provided by RefSeq]. Vogel SN KA and Fenton MJ. TLRs: differential adapter utilization by toll-like receptors mediates TLR-specific patterns of gene expression. Mol. Interv. 2003; 3:466-77. Takeda K Kaisho T and Akira S. Toll-like receptors. Annu. Rev. Immunol. 2003; 21:335-76. Janeway CA Jr. and Medzhitov R. Innate immune recognition. Annu. Rev. Immunol. 2002; 20:197-216. O?Neill LAJ FA and Bowie AG. The Toll-IL-1 receptor adaptor family grows to five members. Trends in Imm. 2003; 24:286-9. McGettrick AF and O?Neill LAJ. The expanding family of MyD88-like adaptors in Toll-like receptor signal transduction. Mol Imm. 2004; 41:577-82. Ochoa M-T Legaspi AJ Hatziris Z et al. Distribution of Toll-like receptor 1 and Toll-like receptor 2 in human lymphoid tissue. Immunol. 2003; 108:10-15. Takeuchi O Sato S Horiuchi T et al. Cutting edge: role of Toll-like receptor 1 in mediating immune response to microbial lipoproteins. J. Immunol. 2002; 169:10-4.