Anti-PUMA (p53 Upregulated Modulator of Apoptosis)

GWB-D0A9EC

This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids near the carboxyl terminus of the human PUMA protein. The qimmunogen sequence is identical between the ? and ? forms of the PUMA protein. Family: ApoptosisFraction: Affinity Purified

ELISA User OptimizedWESTERN BLOT 1-2 ?g/mlIMMUNOCYTOCHEMISTRY 1 ?g/mlOTHER APPLICATIONS User OptimizedPhysical State: Liquid (sterile filtered)

This affinity purified antibody is directed against human PUMA protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. The antibody also recognizes mouse PUMA protein. Reactivity against homologues from other sources has not been determined.

Dry Ice Apoptosis is related to development as well as to many diseases. The p53 tumorsuppressorprotein induces apoptosis through transcriptional activation of several genes. A novel p53 induciblepro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis)and bbc3 (for Bcl-2 binding component 3) in human and mouse (1-3). PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members. This family of proteins also includes Bax and Noxa which are also transcriptional targets ofp53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-? and PUMA-? (1). PUMAproteins bind Bcl-2 localize to the mitochondria and induce cytochrome c release and apoptosis in response top53. PUMA may be a direct mediator of p53-induced apoptosis. N/A 1. Nakano K Vousden KH (2001) PUMA a novel proapoptotic gene is induced by p53. Mol. Cell 7:683-694. 2. Yu J Zhang L Hwang PM Kinzler KW Vogelstein B (2001) PUMA induces the rapid apoptosis of colorectalcancer cells. Mol. Cell 7:673-682. 3. Han J Flemington C Houghton AB Gu Z Zambetti GP Lutz RJ Zhu L Chittenden T (2001) Expression ofbbc3 a pro-apoptotic BH3-only gene is regulated by diverse cell death and survival signals. Proc. Natl. Acad. Sci. USA 98:11318-11323.