RABBIT ANTI RIG-I

RIG-I

Phosphate buffered saline

Human GST-tagged RIG-1 protein.

Involved in innate immune defense against viruses. Upon interaction with intracellular dsRNA produced during viral replication triggers a transduction cascade involving MAVS/IPS1 which results in the activation of NF-kappa-B IRF3 and IRF7 and the induction of the expression of antiviral cytokines such as IFN-beta and RANTES (CCL5). Detects dsRNA produced from non-self dsDNA by RNA polymerase III such as Epstein-Barr virus-encoded RNAs (EBERs). Essential for the production of interferons in response to RNA viruses including paramyxoviruses influenza viruses Japanese encephalitis virus and HCV By similarity. Ref. 9Ref. 10Ref. 12Ref. 13Ref. 14Ref. 17Ref. 18Ref. 20Ref. 22Catalytic activityATP + H2O = ADP + phosphate. Cofactor: Zinc. Subunit structureMonomer; maintained as a monomer in an autoinhibited state. Upon viral dsRNA binding and conformation shift homomultimerizes and interacts with MAVS. Interacts with DHX58/LGP2 IKBKE TBK1 and TMEM173/STING. Interacts (via CARD domain) with TRIM25 (via SPRY domain). Interacts with RNF135. Interacts with CYLD. Ref. 10Ref. 13Ref. 14Ref. 17Ref. 18Ref. 11Ref. 16Ref. 19Ref. 21Ref. 23Subcellular locationCytoplasm.

The repressor domain controls homomultimerization and interaction with MAVS Probable. Ref. 17The helicase domain is responsible for dsRNA recognition. Ref. 17The 2 CARD domains are responsible for interaction with and signaling through MAVS. Ref. 17The second CARD domain is the primary site for \' Lys-63\' -linked ubiquitination. Ref. 17Post-translational modificationIsgylated. Conjugated to ubiquitin-like protein ISG15 upon IFN-beta stimulation. Ubiquitinated. Undergoes \' Lys-63\' -linked ubiquitination. Lys-172 is the critical site for TRIM25-mediated ubiquitination for MAVS binding and to induce anti-viral signal transduction. Lys-154 Lys-164 and Lys-172 are critical sites for RNF135-mediated ubiquitination. Deubiquitinated by CYLD a protease that selectively cleaves \' Lys-63\' -linked ubiquitin chains. Ref. 18Ref. 16Ref. 21Ref. 23Sequence similaritiesBelongs to the helicase family. Contains 2 CARD domains. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain. 1. Stefanova I. et al. (1989) in Leucocyte Typing IV: White cell differentiation antigens. Ed. Knapp W. et al. Oxford University Press pp 678-697. 2. Stefanova I. et al. (1989) Characterisation of a broadly expressed human leucocyte surface antigen MEM-43 anchored in membrane through phosphotidylinositol. Mol. Immunol. 26: 153 - 161. 3. Tandon N. et al. (1994) . Expression and function of multiple regulators of complement activation in autoimmune thyroid disease. Immunology 81: 643-647. 4. Horejsi V. et al. (1988) Monoclonal antibodies against human leucocyte antigens. II. Antibodies against CD45 (T200) CD3 (T3) CD43 CD10 (CALLA) transferrin receptor (T9) a novel broadly expressed 18-kDa antigen (MEM-43) and a novel antigen of restricted expression (MEM-74). Folia. Biol. (Prague) 34: 23-24. 5. Stefanova I. et al. (1991) Association of the CD59 and CD55 cell surface glycoproteins with other membrane molecules. J. Immunol. 147: 1587 - 1592. [1] \" CD59 an LY-6-like protein expressed in human lymphoid cells regulates the action of the complement membrane attack complex on homologous cells. \" Davies A. Simmons D. L. Hale G. Harrison R. A. Tighe H. Lachmann P. J. Waldmann H. J. Exp. Med. 170:637-654(1989) [PubMed: 2475570] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: T-cell. [2] \" The CD59 antigen is a structural homologue of murine Ly-6 antigens but lacks interferon inducibility. \" Philbrick W. M. Palfree R. G. E. Roger G. E. Maher S. E. Bridgett M. M. Sirlin S. Bothwell A. L. M. Eur. J. Immunol. 20:87-92(1990) [PubMed: 1689664] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA]. [3] \" 20 KDa homologous restriction factor of complement resembles T cell activating protein. \" Okada H. Nagami Y. Takahashi K. Okada N. Hideshima T. Takizawa H. Kondo J. Biochem. Biophys. Res. Commun. 162:1553-1559(1989) [PubMed: 2475111] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA]. [4] \" Molecular cloning and characterization of MACIF an inhibitor of membrane channel formation of complement. \" Sugita Y. Tobe T. Oda E. Tomita M. Yasukawa K. Yamaji N. Takemoto T. Furuichi K. Takayama M. Yano S. J. Biochem. 106:555-557(1989) [PubMed: 2606909] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA]. [5] \" Isolation and expression of the full-length cDNA encoding CD59 antigen of human lymphocytes. \" Sawada R. Ohashi K. Anaguchi H. Okazaki H. Hattori M. Minato N. Naruto M. DNA Cell Biol. 9:213-220(1990) [PubMed: 1692709] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA]. [6] \" Structure of the CD59-encoding gene: further evidence of a relationship to murine lymphocyte antigen Ly-6 protein. \" Petranka J. G. Fleenor D. E. Sykes K. Kaufman R. E. Rosse W. F. Proc. Natl. Acad. Sci. U. S. A. 89:7876-7879(1992) [PubMed: 1381503] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. [7] \" Gene structure of human CD59 and demonstration that discrete mRNAs are generated by alternative polyadenylation. \" Tone M. Walsh L. A. Waldmann H. J. Mol. Biol. 227:971-976(1992) [PubMed: 1383553] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Blood. [8] \" Cloning of human full-length CDSs in BD Creator(TM) system donor vector. \" Kalnine N. Chen X. Rolfs A. Halleck A. Hines L. Eisenstein S. Koundinya M. Raphael J. Moreira D. Kelley T. LaBaer J. Lin Y. Phelan M. Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databasesCited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. [9] \" The status quality and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). \" The MGC Project TeamGenome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Colon. [10] \" Complementary DNA sequence and deduced peptide sequence for CD59/MEM-43 antigen the human homologue of murine lymphocyte antigen Ly-6C. \" Sawada R. Ohashi K. Okano K. Hattori M. Minato N. Naruto M. Nucleic Acids Res. 17:6728-6728(1989) [PubMed: 2476718] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 27-128.