p53

p53

Synthetic phosphopeptide corresponding to an amino acid sequence within p53 which includes phosphorylated Ser392.

Antisera to human phosphorylated p53 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.

Western Blotting - 1:1000

Binds DNA as a homotetramer. Found in a complex with CABLES1 and TP73. Interacts with histone acetyltransferases EP300 and methyltransferases HRMT1L2 and CARM1 and recruits them to promoters. Interacts with ING4 and this interaction may be indirect. The C-terminus interacts with TAF1 when TAF1 is part of the TFIID complex. Interacts with P53DINP1. Interacts with WWOX (By similarity). Interacts with HIPK1 HIPK2 and AXIN1. Probably part of a complex consisting of TP53 HIPK2 and AXIN1. Interacts with USP7 and SYVN1. Interacts with ARMD10 and HSP90AB1 (By similarity). Interacts with BANP CDKN2AIP and E4F1.

The [KR]-[STA]-K motif is specifically recognized by the SETD7 methyltransferase (By similarity).

Acetylated. Its deacetylation by SIRT1 impairs its ability to induce proapoptotic program and modulate cell senescence.

Monomethylated at Lys-366 by SETD7 leading to stabilize it and increase transcriptional activation. Monomethylated at Lys-364 by SMYD2 leading to decrease DNA-binding activity and subsequent transcriptional regulation activity. Lys-366 monomethylation prevents the interaction with SMYD2 and subsequenct monomethylation at Lys-364 (By similarity).

Belongs to the p53 family. 1. Jaiswal. J. et al. (2001) p53-dependent transcriptional regulation of the APC promoter in colon cancer cells treated with DNA alkylating agents.