Caspase-7

GWB-A1D735

A synthetic peptide corresponding to residues near N-terminus of human Caspase-7 was used as immunogen. The antibody should recognize both pro-form and p20 cleaved-form. The antibody does not cross-react with other Caspase family members.

WB: 1:500-1000IHC: 1:50-100ICC: 1:100IP: 1:10. Caspases are a family of cytosolic aspartate-specific cysteine proteases involved in the initiation and execution of apoptosis. Caspase-7(CMH-1 Mch3 ICE-LAP3) is an effector caspase involved in the activation cascade for apoptosis execution (1 2 3). Pro-Caspase-7 is a 35-kDa protein localized to the cytoplasm and expressed in a variety of tissue and cell lines(1). Upon activation Caspase-7 is translocated to the mitochondria and proceeds to proteolytically cleave poly(ADP-ribose) polymerase (PARP)(2 3) and endoplasmic reticular-specific substrate sterol regulatroy element-binding protein 1(4). 1. Duan H. et al. J. Biol. Chem. 271: 1621-5 (1996)2. Lippke J. A. et al. J. Biol. Chem. 271: 1825-8 (1996)3. Fernandes-Alnemri T. et al. Cancer Res. 55: 6045-52 (1995)4. Chandler J. M. et al. J. Biol. Chem. 273: 10815-10818 (1998)5. Manufactured under U. S. Patents No. 5 675 063 and 7 429 487