PARP-1 (p25)

GWB-238759

A synthetic peptide corresponding to residues before the cleavage site of human PARP-1 was used as immunogen. The antibody only recognize p25 cleaved-form of PARP-1. Predicted to cross-react with Chinese hamster based on sequence homology.

WB: 1:50 000 - 100 000IHC: 1:100ICC: 1:250FC: 1:50IP: 1:50. Poly (ADP-ribose) polymerase (PARP) is zinc-dependent DNA binding protein that recognizes DNA strand breaks and is presumed to play a role in DNA repair (1). As a marker for apoptosis PARP is cleaved in vitro by many caspases and in vivo by Caspase-3 (2 3). Existing as a 116 kDa nuclear protein PARP is cleaved between amino acids Asp214 and Gly215 to yield two fragments of 29 kDa (C-terminal catalytic domain) and 85 kDa (N-terminal DNA-binding domain) (2 4). 1. Bai Y Meng Z Cui M et al An Ang1-Tie2-PI3K axis in Neural Progenitor Cells Initiates Survival Responses Against Oxygen And Glucose Deprivation Neuroscience 160 (2009) 371-381[Application: WB]2. Hong Z Zhang QY Liu J et al Phosphoproteome Study Reveals Hsp27 as a Novel Signaling Molecule Involved in GDNF-Induced Neurite Outgrowth J. Proteome Res. 8 (2009) 2768-2787[Application: WB]3. Gorodetsky E Calkins S Ahn J et al ATM the Mre11/Rad50/Nbs1 complex and topoisomerase I are concentrated in the nucleus of Purkinje neurons in the juvenile human brain DNA repair 6 ( 2007 ) 1698-1707[Application: ICC]4. Dittmer A Hohlfeld K LÃ ¼ tzkendorf J et al Human mesenchymal stem cells induce E-cadherin degradation in breast carcinoma spheroids by activating ADAM10 Cell Mol Life Sci 66 (2009) 3053-3065[Application: WB]5. Bai Y Meng Z Cui M et al An Ang1-Tie2-PI3K axis in Neural Progenitor Cells Initiates Survival Responses Against Oxygen And Glucose Deprivation Neuroscience 160 (2009) 371-381[Application: WB]1. Ikejima M et al. The zinc fingers of human poly(ADP-ribose) polymerase are differentially required for the recognition of DNA breaks and nicks and the consequent enzyme activation. Other structures recognize intact DNA. J. Biol. Chem. 265: 219072. Lazebnik Y. A. et al. Cleavage of poly(ADP-ribose) polymerase by a proteinase with properties like ICE. Nature 371: 3463. Tewari M et al. Yama/CPP32 beta a mammalian homolog of CED-3 is a CrmA-inhibitable protease that cleaves the death substrate poly(ADP-ribose) polymerase. Cell 81: 8014. Kaufmann S. H. et al. Specific proteolytic cleavage of poly(ADP-ribose) polymerase: An early marker of chemotherapy-induced apoptosis. Cancer Res. 53: 29765. Manufactured under U. S. Patents No. 5 675 063 and 7 429 487