Cdc25C

GWB-257318

A synthetic peptide corresponding to residues near N-terminus of human Cdc25C was used as immunogen. The antibody can also detect splice isoform 2 4 and 5 of human Cdc25C based on sequence homology.

WB: 1:5 000IHC: 1:250-500ICC: 1:250IP: 1:80. Cdc25C is a tyrosine protein phosphatase required for progression of the cell cycle. Cdc25C activates cell cycle-specific cyclin-dependent kinases (CDKs) including cdc2 via dephosphorylation events (1 2). Cdc25C is activated via phosphorylation at Ser216 (3). In response to DNA damage and DNA replicational stress Chk1 and Chk2 phosphorylate Cdc25C to prevent entry into mitosis (4 5). 1. Cui J Xing L Li Z et al Ochratoxin A induces G2 phase arrest in human gastric epithelium GES-1 cells in vitro Toxicol Lett 193 (2010) 152-158[Application: WB]1. Sadhu K. et al. Human homolog of fission yeast cdc25 mitotic inducer is predominantly expressed in G2. Proc Natl Acad Sci U S A. 87: 51392. Jessus C. and R. Ozon. Function and regulation of cdc25 protein phosphate through mitosis and meiosis. Prog. Cell Cycle Res. 1: 2153. Peng C. Y. et al. Mitotic and G2 checkpoint control: regulation of 14-3-3 protein binding by phosphorylation of Cdc25C on serine-216. Science 277: 15014. Blasina A. et al. A human homologue of the checkpoint kinase Cds1 directly inhibits Cdc25 phosphatase. Curr Biol. 9: 15. Matsuoka S. et al. Linkage of ATM to cell cycle regulation by the Chk2 protein kinase. Science 282: 18936. Manufactured under U. S. Patents No. 5 675 063 and 7 429 487