Active Caspase-3

GWB-FD72D6

A synthetic peptide corresponding to residues following Ser29 of human caspase-3 (N-terminus of p17 subunit) was used as immunogen. This antibody only detects the active (cleaved) form of Caspase-3 and does not recognize the pro form of Caspase-3.

WB: 1:1000 - 10 000IHC: 1:250ICC: 1:100-250. Caspases are a family of cytosolic aspartate-specific cysteine proteases involved in the initiation and execution of apoptosis. Caspase-3 (apopain SCA-1 Yama and CPP32) is a member of the apoptosis execution functional group of caspases and is either partially or totally responsible for the proteolytic cleavage of many key proteins during apoptosis such as poly (ADP-ribose) polymerase (PARP) (1 2 3). Caspase-3 is a cytosolic protein found in cells as an inactive 32 kDa proenzyme. It is activated by proteolytic cleavage into two active subunits only when cells undergo apoptosis (3). 1. Chen C Zhuang XW Epsin 1 is a cargo-specific adaptor for the clathrin-mediated endocytosis of the influenza virus Proceedings of the National Academy of Sciences 105 (2008) 11790-11795[Application: ICC]2. Tugizov S Herrera R Veluppillai P et al Epstein-Barr Virus (EBV)-Infected Monocytes Facilitate Dissemination of EBV within the Oral Mucosal Epithelium Journal of Virology 81 (2007) 5484-5496[Application: ]3. Harrell JC Dye WW Harvell DME et al Estrogen Insensitivity in a Model of Estrogen Receptor-Positive Breast Cancer Lymph Node Metastasis Cancer Res 67 (2007) 10582-10591[Application: IHC]4. Caldon CE Sergio CM Schà ¼ tte J et al Estrogen Regulation of Cyclin E2 Requires Cyclin D1 but Not c-Myc Mol. Cell. Biol. 29 (2009) 4623 - 4639[Application: IP]5. Son M Kim J Han H et al Expression profiles of protein tyrosine kinase genes in human embryonic stem cells Reproduction 136 (2008) 423-432[Application: WB]6. Liu XB Yamada N Maruyama W et al Formation of Dopamine Adducts Derived From Brain Ployunsaturated Fatty Acids: Mechanism for Parkinson\' s Disease J. Biol. Chem. 283 (2008) 34887 - 34895[Application: WB]7. Cangemi R Mensah A Albertini V et al Reduced expression and tumor suppressor function of the ETS transcription factor ESE-3 in prostate cancer Oncogene 27 (2008) 2877-2885[Application: WB]8. Sikora J Harzer K Elleder M Neurolysosomal pathology in human prosaposin deWciency suggests essential neurotrophic function of prosaposin Acta Neuropathol 113 (2007) 163-175[Application: IHC]9. Meng XL Shen JS Kawagoe S et al Induced pluripotent stem cells derived from mouse models of lysosomal storage disorders Proc Natl Acad Sci U S A 107 (2010) 7886-7891[Application: WB]1. Fernandes-Alnemri T. et al. J. Biol. Chem. 269:30761-4 19942. Tewari M. et al. Cell 81: 801-9 19953. Nicholson D. W. et al. Nature 376: 37-43 19954. Manufactured under U. S. Patents No. 5 675 063 and 7 429 487