GFP (N-term)

GWB-85660E

A synthetic peptide corresponding to residues in the N-terminus of Aequorea victoria green fluorescent protein was used as immunogen. The antibody recognizes GFP and GFP fusion proteins.

WB: 1:1 000 - 10 000. The green fluorescent protein (GFP) from the Pacific Northwest jellyfish Aequorea victoria (1) is extensively used as a fluorescent marker in molecular and cell biology both in vitro and in vivo. The protein is in the shape of a cylinder comprising 11 strands of beta-sheet with an alpha-helix inside and short helical segments on the ends of the cylinder. This motif with beta-structure on the outside and alpha-helix on the inside is also referred as the beta-can (2-3). This rabbit monoclonal antibody can recognize wild type as well as the humanized formed of GFP and is especially useful for working with GFP expressed in Rodent cell lines or tissues. 1. Kumar N Afeyan R Kim HD et al Multipathway Model Enables Prediction of Kinase Inhibitor Cross-Talk Effects on Migration of Her2-Overexpressing Mammary Epithelial Cells Mol Pharmacol 73 (2008) 1668-1678[Application: ICC]2. Bouhidel O Pons S Souktani R et al Myocardial ischemic postconditioning against ischemia-reperfusion is impaired in ob/ob mice Am J Physiol Heart Circ Physiol 295 (2008) H1580-H1586[Application: WB]3. LeBrasseur NK Schelhorn TM Bernardo BL et al Myostatin Inhibition Enhances the Effects of Exercise on Performance and Metabolic Outcomes in Aged Mice J Gerontol A Biol Sci Med Sci 64A (2009) 940 - 948[Application: WB]4. Aoudjit L Zou YL Liu XW et al Nck Proteins Maintain the Adult Glomerular Filtration Barrier J. Am. Soc. Nephrol. 20 (2009) 1533 - 1543[Application: ]5. Wan M Qin J Songyang Z et al OB Fold-containing Protein 1 (OBFC1) a Human Homolog of Yeast Stn1 Associates with TPP1 and Is Implicated in Telomere Length Regulation J Biol Chem. 284 (2009) 26725-26731[Application: WB]6. Liu CK Meng CM Xie L et al Cell-to-cell trafficking subcellular distribution and binding to coat protein of Broad bean wilt virus 2 VP37 protein Virus Res 143 (2009) 86-93[Application: WB]7. Xiong RY Wu JX Zhou YJ et al Characterization and subcellular localization of an RNA silencing suppressor encoded by Rice stripe tenuivirus Virology 387 (2009) 29-40[Application: WB]8. Shi J Chen WW Liu Q et al Depletion of the MobB and CotA complex in Aspergillus nidulans causes defects in polarity maintenance that can be suppressed by the environment stress Fungal Genetics and Biology 45 (2008) 1570-1581[Application: WB]9. Mallaun M Naeher D Daniels MA et al The T Cell Receptorâ ??s -Chain Connecting Peptide Motif Promotes Close Approximation of the CD8 Coreceptor Allowing Efficient Signal Initiation The Journal of Immunology 180 (2008) 8211-8221[Application: IP]10. Mallaun M Naeher D Daniels MA et al GFP J Immunol 180 (2008) 8211-8221[Application: IP]11. Chen S Song Y Cao J et al Localization and function of calmodulin in live-cells of Aspergillus nidulans Fungal Genet Biol 47 (2010) 268-278[Application: WB]12. Choi YB Nicholas J Induction of angiogenic chemokine CCL2 by human herpesvirus 8 chemokine receptor Virology 397 (2010) 369-378[Application: WB ICC]13. He B Yu X Margolis M et al Live-Cell Imaging in Caenorhabditis elegans Reveals the Distinct Roles of Dynamin Self-Assembly and Guanosine Triphosphate Hydrolysis in the Removal of Apoptotic Cells Mol Biol Cell 21 (2010) 610-6291. Prasher D. C. Eckenrode V. K. Ward W. W. Prendergast F. G. Cormier M. J. ; Primary structure of the Aequorea victoria green-fluorescent protein. ; Gene 111:229-233(1992). 2. Cody C. W. Prasher D. C. Westler W. M. Prendergast F. G. Ward W. W. ; Chemical structure of the hexapeptide chromophore of the Aequorea green-fluorescent protein. ; Biochemistry 32:1212-1218(1993). 3. Ormoe M. Cubitt A. B. Kallio K. Gross L. A. Tsien R. Y. Remington S. J. ; Crystal structure of the Aequorea victoria green fluorescent protein. ; Science 273:1392-1395(1996). 4. Manufactured under U. S. Patents No. 5 675 063 and 7 429 487