TSC2 C-term

GWB-70BF38

A synthetic peptide corresponding to residues in the C-term of human TSC2 was used as an immunogen. Predicted to detect isoform 2-4 based on sequence homology.

WB: 1:200-1000IHC: 1:50FC: 1:50. Tuberous sclerosis complexe-2 (TCS2 also known as Tuberin) is a tumor suppressor that form a complex with TSC1 (Hamartin ) and this complex is known to control various cellular functions including cell cycle endocytosis adhesion and transcription (1). The C-terminal region of TSC2 contains a GTPase-activating protein (GAP) domain which interacts with Rap1 Rab5 and Rheb (2). The TSC1/TSC2 complex inhibits phosphorylation of S6kinase and 4E-BP1 through inactivation of mTOR (3). Additionally binding of 14-3-3 beta to TSC2 at Ser1210 reduce the ability of the complex to inhibit phosphorylation of S6 kinase (4). Phosphorylation by ATK at Ser924 and Thr1518 inactivates TSC2 and disrupts its interaction with TSC1 (5). Tuberous sclerosis (TSC) an autosomal dominant disorder that affects 1 in 6000 individuals is caused by a mutation in either the TSC1 or TSC2 tumor suppressor gene. 1. Klein UR Haindl M Nigg EA et al RanBP2 and SENP3 function in a mitotic SUMO2/3 conjugation-deconjugation cycle on Borealin Mol. Biol. Cell 20 (2009) 410 - 418[Application: WB]2. Horwitz KB Dye WW Harrell JC et al Rare steroid receptor-negative basal-like tumorigenic cells in luminal subtype human breast cancer xenografts PNAS 105 (2008) 5774-5779 [Application: IHC]3. Nery FC Zeng J Niland BP et al TorsinA binds the KASH domain of nesprins and participates in linkage between nuclear envelope and cytoskeleton J Cell Sci 121 (2008) 3476-3486[Application: WB]4. Kim WY Chang DJ Hennessy B et al A Novel Derivative of the Natural Agent Deguelin for Cancer Chemoprevention and Therapy Cancer Prev Res 1 (2008) 577-587[Application: WB]5. Blando J Portis M Benavides F et al PTEN Deficiency Is Fully Penetrant for Prostate Adenocarcinoma in C57BL/6 Mice via mTOR-Dependent Growth Am J Pathol 174 (2009) 1869-1879[Application: WB]6. Alexander A Cai SL Kim J et al ATM signals to TSC2 in the cytoplasm to regulate mTORC1 in response to ROS Proc Natl Acad Sci U S A 107 (2010) 4153-4158[Application: WB]7. Dere R Wilson PD Sandford RN et al Carboxy Terminal Tail of Polycystin-1 Regulates Localization of TSC2 to Repress mTOR PLoS One 5 (2010) e9239[Application: WB]1. Lamb et al. Nature Cell Biol. 2: 281-2872. Xiao et al J. Biol. Chem. 272: 6097-61003. Manning B. D. ; Tee A. R. ; Logsdon M. N. ; Blenis J. ; Cantley L. C. : Molec. Cell 10: 151-162 2002. 4. Shumway S. D. ; Li Y. ; Xiong Y. : J. Biol. Chem. 278: 2089-2092 2003. 5. Inoki K. ; Li Y. ; Zhu T. ; Wu J. ; Guan K. -L. : 4: 648-657 2002. 6. Manufactured under U. S. Patents No. 5 675 063 and 7 429 487