Chk1 (C-term)

GWB-8158EA

A synthetic peptide corresponding to C-terminal residues of human Chk1 was used as an immunogen.

WB: 1:10 000IHC: 1:250 - 500ICC: 1:100 - 250FC: 1:160. Chk1 is involved in cell cycle arrest when DNA damage has occurred and it plays an important role in DNA damage checkpoint embryonic development and tumor suppression (1). Chk1 is activated in response to replication blocks and genotoxic stress via phosphorylation of serines 317 and 345 (2). Activated Chk1 can bind to phosphorylate (at serine 216) and inactivate cdc25c (3 4). Phosphorylation of cdc25c prevents activation of the CDC2-cyclin B complex and prevents entry into M-phase (3 4). 1. Bekeredjian-Ding I Doster A Schiller M et al TLR9-Activating DNA Up-Regulates ZAP70 via Sustained PKB Induction in IgM+ B Cells J Immunol 181 (2008) 8267-8277[Application: WB]2. Verlinden L Bempt IV Eelen G et al The E2F-Regulated Gene Chk1 Is Highly Expressed in Triple-Negative Estrogen Receptor /Progesterone Receptor /HER-2 Breast Carcinomas Cancer Res 67 (2007) 6574-6481[Application: IHC]1. Martinho RG et al. EMBO J. 17:7239-7249 1998. 2. Zhao H et al. Mol Cell Biol 21:4129-4139 2001. 3. Zeng Y et al. Nature 395:507-510 1998. 4. Sanchez Y et al. Science 277:1497-1501 1997. 5. Manufactured under U. S. Patents No. 5 675 063 and 7 429 487