Hsp70 (C-term)

GWB-ACD7D3

A synthetic peptide corresponding to residues near the C-term of human Hsp70 was used as an immunogen.

WB: 1:1 000IHC: 1:50 - 100FC: 1:10IP: 1:50. The 70-kDa Heat Shock Protein (Hsp70) is a highly conserved member of the molecular chaperone network commonly found in nuclear cytosolic mitochondrial and endoplasmic reticulum compartments of all eukaryotic and prokaryotic cells. Hsp70 uses ATP binding and hydrolysis to assist in efficient folding of both newly created and stress-denatured polypeptides. (1). Under environmental stress conditions synthesis of Hsp70 is quickly up-regulated and redistributed throughout the cell. (2). It has been shown that Hsp70 has the ability to bind with high affinity to the plasma membrane activate NF-KappaB and also up-regulate expression of pro-inflammatory cytokines tumor necrosis factor (TNF)-a interleukin (IL)-1beta and IL-6 in human monocytes (3). 1. Zhang C Wang Y Zhou Z et al Sodium butyrate upregulates expression of NKG2D ligand MICA/B in HeLa and HepG2 cell lines and increases their susceptibility to NK lysis Cancer Immunol Immunother 58 (2009) 1275-1285[Application: WB]1. Yam AY et al. J Biol Chem 50:41252-61 20052. Prohaszka Z et al. Cell Stress Chaperones 1:17-22 20023. Asea A. J Biol Chem 17:15028-34 20024. Manufactured under U. S. Patents No. 5 675 063 and 7 429 487