NOTCH1

GWB-A6E1C4

A synthetic peptide corresponding to the Cytoplasmic portion of human Notch1 was used as immunogen.

WB: 1:1 000 - 10 000IHC: 1:50 -100ICC: 1:100-250. Notch (hN1 Motch in mouse Xotch in Xenopus) is a family of large transmembrane receptors involved in cell to cell interactions and cell fate decisions during invertebrate and vertebrate development. A modification of Notch signaling pathway can lead to changes in cellular proliferation differentiation and apoptotic events (1). Notch is activated by binding to membrane-bound ligands of the Delta and Serrate/Jagged family. Activated Notch receptor is proteolytically cleaved and the Notch intracellular domain (NICD) is released. The NICD binds to CSL DNA-binding protein and the Mastermind/Lag-3 co-activator in the nucleus which activates target gene expression (2 3). Notch has been implicated in T-cell acute lymphoblastic leukemia (T-All) and Multiple Sclerosis (MS). Also repression of Notch signaling is commonly seen in cancer (4). In mammals four different Notch receptors has been identified (Notch 1 2 3 and 4). Active Notch1 has been linked to inactivation of p53-dependent transactivation by inhibiting the phosphorylation of p53 protein (5). 1. Frechet M Warrick E Vioux C et al Overexpression of matrix metalloproteinase 1 in dermal fibroblasts from DNA repair- eficient/ cancer-prone xeroderma pigmentosum group C patients Oncogene 27 (2008) 5223-5232[Application: WB]2. Firestein R Bass AJ Kim SY et al CDK8 is a colorectal cancer oncogene that regulates Î ² -catenin activity Nature 455 (2008) 547- 553[Application: WB]3. Ma J Meng Y Kwiatkowski DJ et al Mammalian target of rapamycin regulates murine and human cell differentiation through STAT3/p63/Jagged/Notch cascade J Clin Invest 120 (2010) 103-114[Application: WB]1. Cormier S et al. MCB 26(9):3541-3549 2006. 2. Mumm JS and R Kopan Dev. Biol. 228:151-165 2000. 3. Schweisguth F Curr. Biol. 14:R129-R138 2004. 4. Joutel A et al. Semin Cell Dev Biol 9(6):619-25 1998. 5. Kim SB et al. Cell Death Differ. 20066. Manufactured under U. S. Patents No. 5 675 063 and 7 429 487