SIRT2 (C-term)

GWB-190F3A

A synthetic peptide corresponding to residues on the C-terminus of human SIRT2 was used as an immunogen.

WB: 1:1000ICC: 1:100 - 250FC: 1:30IP: 1:50. Silent information regulator 2 (Sir2) family of enzymes has been implicated in many cellular processes that include histone deacetylation gene silencing chromosomal stability and aging. O-acetyl-ADP-ribose causes a delay/block in oocyte maturation and results in a delay/block in embryo cell division in blastomeres. It has been demonstrated that the production of O-acetyl-ADP-ribose is evolutionarily conserved among Sir2-like enzymes from yeast Drosophila and human. Also endogenous yeast Sir2 complex from telomeres was shown to generate O-acetyl-ADP-ribose (1). SIRT2 is a predominantly cytoplasmic protein that colocalizes with microtubules. SIRT2 deacetylates lysine-40 of alpha-tubulin both in vitro and in vivo. Knockdown of SIRT2 via siRNA results in tubulin hyperacetylation. SIRT2 colocalizes and interacts in vivo with HDAC6 another tubulin deacetylase (2). Cytoskeleton-related protein SIRT2 is down-regulated in gliomas and data suggests that ectopic expression of SIRT2 in glioma cell lines led to the perturbation of the microtubule network (3). 1. Borra MT et al. J Biol Chem 277(15):12632-41 2002. 2. North BJ et al. Mol Cell 11(2):437-44 2003. 3. Hiratsuka M et al. Biochem Biophys Res Commun 309(3):558-66 2003. 4. Manufactured under U. S. Patents No. 5 675 063 and 7 429 487