Mo25

GWB-8AC980

A synthetic peptide corresponding to residues on the C-terminus of human Mo25 was used as an immunogen.

WB: 1:1 000 - 5 000IHC: 1:100 - 250ICC: 1:100 - 250FC: 1:25IP: 1:50. Mouse protein 25 alpha (MO25 alpha) is a 40-kDa protein that together with the STE20-related adaptor-alpha (STRAD alpha) pseudo kinase forms a regulatory complex capable of stimulating the activity of the LKB1 tumor suppressor protein kinase. The latter is mutated in the inherited Peutz-Jeghers cancer syndrome (PJS). MO25 alpha binds directly to a conserved Trp-Glu-Phe sequence at the STRAD alpha C terminus markedly enhancing binding of STRAD alpha to LKB1 and increasing LKB1 catalytic activity (1). Skeletal muscle contraction results in the phosphorylation and activation of the AMP-activated protein kinase (AMPK) by an upstream kinase (AMPKK). The LKB1-STE-related adaptor (STRAD)-mouse protein 25 (MO25) complex is the major AMPKK in skeletal muscle; however LKB1-STRAD-MO25 activity is not increased by muscle contraction. This relationship suggests that phosphorylation of AMPK by LKB1-STRAD-MO25 during skeletal muscle contraction may be regulated by allosteric mechanisms (2). 1. Gast D Riedle S Issa Y et al The cytoplasmic part of L1-CAM controls growth and gene expression in human tumors that is reversed by therapeutic antibodies Oncogene 27 (2008) 1281-1289[Application: WB]2. Xie ZL Dong YZ Zhang JH et al Identification of the Serine 307 of LKB1 as a Novel Phosphorylation Site Essential for Its Nucleocytoplasmic Transport and Endothelial Cell Angiogenesis Mol Cell Biol. 29 (2009) 3582-3596[Application: WB]1. Milburn CC et al. Nat Struct Mol Biol 11(2):193-200 20042. Ellingson WJ et al. Am J Physiol Endocrinol Metab 292(2):400-7 20063. Manufactured under U. S. Patents No. 5 675 063 and 7 429 487